Recombinant Rat Granzyme B (Gzmb)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Gzmb

Uniprot NO.

P18291

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

21-248

Target Protein Sequence

IIGGHEAKPH SRPYMAYLQI MDEYSGSKKC GGFLIREDFV LTAAHCSGSK INVTLGAHNI KEQEKMQQII PVVKIIPHPA YNSKTISNDI MLLKLKSKAK RSSAVKPLNL PRRNVKVKPG DVCYVAGWGK LGPMGKYSDT LQEVELTVQE DQKCESYLKN YFDKANEICA GDPKIKRASF RGDSGGPLVC KKVAAGIVSY GQNDGSTPRA FTKVSTFLSW IKKTMKKS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I would like to confirm that this is specific for rat GZMB?

We compared the above sequences with UniProt database, and the similarity of more than 85% was rat species.
https://www.uniprot.org/blast/uniprotkb/ncbiblast-R20231129-012453-0940-69346677-p1m/overview

Target Background

Function(From Uniprot)

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Subcellular Location

Secreted. Cytolytic granule.

Protein Families

Peptidase S1 family, Granzyme subfamily

Database Links

UNIGENE: Rn.21395

KEGG: rno:100361468

STRING: 10116.ENSRNOP00000056412

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