Recombinant Rat Elongation factor 1-alpha 1 (Eef1a1)

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Eef1a1
Uniprot NO.
Alternative Names
Eef1a1; Eef1aElongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1
Species
Rattus norvegicus (Rat)
Source
Yeast
Expression Region
1-462
Target Protein Sequence
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GSASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK VTKSAQKAQK AK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
full length protein
Tag Info
N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Storage Condition
Store at -20°C, for extended storage, conserve at -20°C or -80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1.
Gene References into Functions
  1. eEF1alpha-cytoskeleton interactions play a role in perinuclear mRNA localization. PMID:16723660
  2. Glutamate exerts an exquisite time-dependent translational control in glial cells, a process that might be critical for glia-neuron interactions in Bergmann glial cells. PMID:20817065
  3. stimulation of translation by nerve growth factor PMID:11907030
  4. eEF1A is enriched in the rat cerebellar postsynaptic density (PSD) fraction and is tightly associated with the PSD 'core', colocalizing with the PSD marker PSD95 in dissociated cerebellar cultures and immunoelectron microscopy of adult rat cerebellum. PMID:15147779
  5. mRNA for eEF1A is present in pyramidal cell dendrites and that the LTP-associated increase in eEF1A expression was intact in dendrites that had been severed from their cell bodies. PMID:15958750
  6. eEF1A is coupled to both inhibitory and excitatory receptors, in a process that may involve the ERK-signaling pathway PMID:17965018
  7. EF and GAPDH were identified as the most stable reference genes in auditory cortex and cochlea, respectively. PMID:18720057
  8. 5-bp internal stem in the metallothionein-1 3'UTR is critical for binding of eEF1alpha and histidine-tRNA ligase, and that binding of eEF1alpha is facilitated through domains I and III. PMID:19501573

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Subcellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane.
Protein Families
TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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