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Recombinant Rat Diacylglycerol kinase zeta (Dgkz), partial

Recombinant Rat Diacylglycerol kinase zeta (Dgkz), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Dgkz

Uniprot NO.

O08560

Alternative Names

Dgkz; Dagk6; Diacylglycerol kinase zeta; DAG kinase zeta; EC 2.7.1.107; 104 kDa diacylglycerol kinase; DGK-IV; Diglyceride kinase zeta; DGK-zeta

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-929

Target Protein Sequence

MEPRDPSPEA RSSDSESASA SSSGSERDAD PEPDKAPRRL TKRRFPGLRL FGHRKAITKS GLQHLAPPPP TPGAPCGESE RQIRSTVDWS ESAAYGEHIW FETNVSGDFC YVGEQYCVAK MLPKSAPRRK CAACKIVVHT PCIGQLEKIN FRCKPSFRES GSRNVREPTF VRHHWVHRRR QDGKCRHCGK GFQQKFTFHS KEIVAISCSW CKQAYHSKVS CFMLQQIEEP CSLGVHAAVV IPPTWILRAR RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL ACGGDGTVGW ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE PVSKILSHVE EGNVVQLDRW DLRAEPNPEA GPEERDDGAT DRLPLDVFNN YFSLGFDAHV TLEFHESREA NPEKFNSRFR NKMFYAGTAF SDFLMGSSKD LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP WGHPGEHHDF EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV DGEPCKLAAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS RVSMHDYEAL HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQQEPD GAGAKSPMCH PLSSKWCFLD ATTASRFYRI DRAQEHLNYV TEIAQDEIYI LDPELLGASA RPDLPTPTSP LPASPCSPTP GSLQGDAALP QGEELIEAAK RNDFCKLQEL HRAGGDLMHR DHQSRTLLHH AVSTGSKEVV RYLLDHAPPE ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDQ QGDTPRQRAE KAQDTELAAY LENRQHYQMI QREDQETAV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol. By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane. Regulates RASGRP1 activity.

Gene References into Functions

The results suggest that DGKzeta cytoplasmic translocation in neurons under hypoxic stress is regulated by some mechanism which differs from that mediated by NAP1-like proteins. PMID:24893663
The DGKzeta and DGKalpha localize predominantly to the nucleus of ganglion cells. PMID:23467923
The results show that DGKzeta cytoplasmic translocation is triggered by activation of NMDAR with subsequent extracellular Ca(2+) influx. PMID:22234382
Results suggest that DGKzeta is involved in the acute phase of cellular response to ischemic stress in cardiomyocytes PMID:21938675
within the cardiomyocyte, there exists a PPAR-DGK signalling axis that underpins the ability of PPAR ligands to inhibit ET1-dependent hypertrophy PMID:21183507
Cytoplasmic translocation of DGKzeta might be involved in early events of the apoptotic cell death pathway in hippocampal neurons under stressed conditions PMID:21362459
DGK zeta is primarily a nuclear protein in neurons, it can also be cytoplasmic in some conditions, and the subcellular location depends not only on the cell type but also on the developmental state or growth conditions of the cell. PMID:14511325
diacylglycerol kinase zeta might be involved in the process of selective vulnerability of hippocampal pyramidal neurons in postischemic brain PMID:15542238
DGK-zeta blocked ET-1-induced activation of the PKC-epsilon-ERK-AP1 signaling pathway, atrial natriuretic factor gene induction, and resultant cardiomyocyte hypertrophy PMID:15781737
In rat DGKzeta-transgenic mice, DGKzeta negatively regulated the hypertrophic signaling cascade and resultant cardiac hypertrophy in response to GPCR agonists PMID:16380548
DGKzeta is induced in activated microglia in brain trauma, suggesting the functional significance of DGKzeta in this process. PMID:19520144