Recombinant Rat Chymase (Cma1)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cma1

Uniprot NO.

P50339

Alternative Names

Cma1; Mcpt3Chymase; EC 3.4.21.39; Alpha-chymase; Mast cell protease 3; rMCP-3; Mast cell protease 5; rMCP-5; Mast cell protease III; rMCP-III

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

22-247

Target Protein Sequence

IIGGTECIP HSRPYMAYLE IVTSDNYLSA CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTYKEDTWQK LEVEKQFIHP NYDKRLVLHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG RTNVNEPASD TLQEVKMRLQ EPQSCKHFTS FQHKSQLCVG NPKKMQNVYK GDSGGPLLCA GIAQGIASYV HPNAKPPAVF TRISHYRPWI NKILREN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Gene References into Functions

The pathobiologic response in WKY rats was directly linked to increases in cardiac gene expression and enzymatic activity of chymase and modest reductions in ACE2 activity. PMID:28748720
Compared to chymase, ACE-mediated angiotensin-(1-12) metabolism in the heart was several fold lower. Overall our findings suggest that angiotensin-(1-12), not Ang I, is the better substrate for Ang II formation by chymase in adult rats. PMID:27465904
In a pure VO model, chymase produced in adult cardiac fibroblasts leads to autophagic degradation of newly synthesized intracellular procollagen I, suggesting a new role of chymase in extracellular matrix degradation. PMID:26807691
The results indicate an important role for chymase in inducing fibrosis through TGF-beta1 activation, parallel with Ang II effects. PMID:22875344
Chymase-secreting mast cells mediate apoptosis of neighboring smooth muscle cells through a mechanism involving degradation of pericellular fibronectin and disruption of the p-focal adhesion kinase-dependent cell-survival signaling cascade. PMID:12588765
rat alpha-chymase has converted to elastase-like substrate specificity, perhaps associated with an adoption of new biological targets PMID:12900423
Based on the results, this study suggested rMCP-5 is a key promoter of angiogenesis in the rat. PMID:15723097
Plasma ANG II does not appear to regulate intrarenal chymase activity. ACE-independent ANG II production by chymase may provide mechanism for augmenting intrarenal ANG II in ischemic kidney in renovascular hypertension. PMID:15869764
By disrupting the NF-kappaB-mediated survival-signaling pathway, activated chymase-secreting mast cells can mediate apoptosis of cultured arterial SMCs. PMID:16460729
Angiotensin II-forming chymase may play a role in the proliferation of the medial layer in the lung arterioles of monocrotaline-induced pulmonary hypertensive rats. PMID:16690960
These results suggest that chymase can promote cardiac fibroblast proliferation and collagen synthesis via TGF-beta 1/Smad pathway rather than angiotensin II, which is implicated in the process of myocardial fibrosis. PMID:18057996
chymase inactivates the FAK-mediated cell survival signaling PMID:18079408