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Recombinant Rat Apolipoprotein B-100 (Apob), partial

Recombinant Rat Apolipoprotein B-100 (Apob), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

Apob

Purity

>85% (SDS-PAGE)

Target Names

Apob

Uniprot NO.

Q7TMA5

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

28-4743

Target Protein Sequence

QDA TRFKHLRKYV YSYEAESSSG VRGTADSRSA TKINCKVELE VPQVCTLIMR TSQCTLKEVY GFNPEGKALM KKTKNSEEFA SAMSRYELKL AFPEGKRVAL YPDLGEPNYI LNIKRGIISA LLVPPETEED KQVLFQDTVY GNCSTQVTVN SRKGTVATEM STERNLQHCD GFQPISTSVS PLALIKGLVR PLSTLISSSQ SCQYTLEPKR KHVSEAICNE QHLFLPFSYK NKYGIMTHVT QKLSLEDTPK INSRFFRGGI NQVGLAFEST KSTSPPKQAD AVLKTLQELK KLSISEQNAQ RANLFHKLVT ELRGLSGEAI TSLLPQLIEV SSPITLQALI QCGQPECYTH ILQWLKTEKA HPLLIDIVTY LMALIPNPSV QRLQEIFNTA KELQSRATLY ALSHAVNSYY AIMDHSRSPV LEDIAGYLMK QIDNECMGDE DRTFLILRVI GNMGRTMERV MPALKSSVLN CVRSTKPSLQ IQKAALQALR KMEMGDEVRT ILFDTFVNDV APVEKRLAAY LLLMRSPSSS DINKIAKLLQ WEQSEQVKNF VASHIANILN SEELYVQDLK NLIKNALVNS RLPTIMDFRK FSRNYQISKS VSIPLFDPVS AKIEGNLVFD PSSYLPKESM LKTTLTVFGI ASLDLFEIGL EGKGFEPTLE ALFGKQGFFP DSVNKALYWV NGQVPDRVSK VLVDHFGYTK DDKHEQDMVN GIMPIVDKLI KELKSKEIPE ARAYLRILGK ELGFVRLQDL QVLGKLLLNG AQTFRGVPQM IVQAIREGSK DDLFLHYIFM ENAFELPTGV GLQLQVSSSG VFTPGIKAGV RLELANIQAE LVAKPSVSLE FVTNMGIIIP DFAKSGVQMN TNFFHESGLE ARVALKAGQL KVIIPSPKRP VKLFSGSNTL HLVSTTKTEV IPPLIENRKS WSTCKPFFTG MNYCTTGAYS NASSTESASY YPLTGDTRYE LELKPTGEVE QYSASATYEL LKEDKSLVDT LKFLVQAEGV QQSEATAMFK YNRRSRTLSS EVLIPGFDVN FGTILRVNDE SSKDKNTYKL ILDIQNKKIT EVSVVGHVSY DKKGDGKVKG VVSIPRLQAE ARSEVHTHWS PTKLLFQMDS SATAYGSTIS KRVAWRYDNE KIEFDWNTGT NVDTKKVASN FPVDLSRYPR MVHEYANGLL DHRVPQTDMT FRHMGSKLIV DHLNGLSELN LPKVGLPDFH IPDNLFLKTD GRVKYTLNKN RIEIDIPLPL GGKSSKDLKV PESVRTPALN FKSVGFHLPS QEVQIPTFTI PKTHQLQVPL LGILDLSTNV YSNLYNWSVS YTGGNTSRDH FSLQAQYRMK ADSVVDLFSY SVQGSGETTY DSKSTFTLSC DGSLHHKFLD SKFKVSHVEK FGNNPVSKGL LTFETSSALG PQMSATVQLD SKKKQHLYVK DIKVDGQFRV FSLYAQGEYG LSYERDSMTG QMSGESNMKF NSTYFQGTNQ IVGMYQDGML SVTSTSDLQD GIFKNTASLK YENYELTLKS DSSGQYENFA ASNKLDMTFS KQSALLRSEH QANYKSLRLV TLLSGSLTSQ GVELNADILG TDKINTGAHK STLKIAQDGV STSATTNLKY SPLLLENELN AELGLSGASM KLSTSGRFKE HHAKFSLDGR AALTEVSLGS IYQAMILGAD SKNVFNFKLS REGLKLSNDM MGSYAEMKLD HTHSLRISGL SLDFFSKMDN IYSGDKFYKQ NFNLQLQPYS FGITLSNDLK YDALVLTNNG RLRLEPLKLN VGGNFKGTYQ NNELKHIYTI SYTDLVVASY RADTVATVQG VEFSHRLNAD IEGLASSVDV TTSYSSDPLH FNNVFRFVLA PFTLGVDTHT SGDGKMSLWG EHTGQMYSKF LLKAEPLALT FSHDYKGSTS HNLLYKNSVS TALEHTLSAL LTPAEQTSSW KFKTSLNDKV YSQEFEAYNT KDKIGIELSG RADLSGLYSP IKVPFFYSEP VNVLNSLEIN DAFDEPREFT IDAVVKYDKN QDVHTISLPF FQSLPDYLER NRRGIISLLE AMKGELQRLS VDQFVRKYRV ALSRLPQQIH DYLNASDWER QVAGAKEKLT SFMENYRITD NDVLIALDSA KINLNEKLSQ LETYAIQFDQ YIRDNYDAQD LKRTIAQIID RIIEKLKMLD EQYHIRVNLA KSIHNLYLFV ENVDLNQISS SGASWIQNVD TKYQIRIQIQ EKLQHLRTQI HNIDIQQLAA ELKQQIEALD VPMHLDQLRT AILFQRISVI IERVKYFVMN LIEDFKVTEK INTFRVIVRE LIEKYEVDRQ IQVLMDKSIE LAHRYSLSEP LQKLSNVLQQ IEIKDYYDKL VGFIDDTVEW IKAVSFKNII EELNRLIDMS VKKLKAFDYH QFVDKTNSKI REMTQRINAE IQALELPQKT EALKLWVEDF KTTVSNSLEK LKDTKVTVVV DWLQDGLAQI KAQFQDALED VRDRIYQMDI QGELERCLSL VSQVYSTVVT YISDWWTLTA KNITDFAEQY STQKWAESVK ALVEQGFIVP EIQTFLGTMP AFEVSLHALQ EANFQTPDFI VPLTDLRIPS IWINFKMLKN VKIPLRFSTP EFTLLNTFRV RSFTIDLLEI KAKIIRTIDQ MLSSELQWPL PEVYLRDLEM VNISLARLSL PDFHVPEITI PEFTIPNVNL KDLQVPDLHI PEFQLPHLSC TTEIPAFGKL HSVLKIQSPL FILDASANIQ NITTSENKAE IVASVTARGE SKFEALNFDF QAQAQFLELN ANPLVLKESV NFSSKHVRME HEGKILVSGK ALEGKSDTVA RLHTEKNTVE FNNGIVVKIN NQFTLDSQTK YFHKLSVPRL DFSSKASLNN EIKTLLEAGH MAWTSSGTGS WNWACPNFSD EGIHSSKISF IVDGPIASFG LSNNINGKHL RVVQKLTSES GFLNYSRFEV ESKVESQHVG SSILTAEGRA LLGDAKAEMT GEHNANLNGK VIGTLKNSLF FSAQPFEITA STNNEGNLKV SFPLKLTGKI DFLNNYALFL SPHAQQASWQ LSTRFNQYKY NQNFSAINNE HNMEASIVMN GDANLDFLNI PLTIPEINLP YTRFTTPLLK DFSIWEETGL KEFLKTTKQS FDLSIKAQYK KNRDKHSVVI PLKMFYEFML NNVNSWDRKF EKVRDNALHF LTASYNETKI KFDKYKTENS LNQPSRTFQN RGHTIPVLNI EVSPFAVETL ASSHVIPKAI RTPSVTIPGP NIIVPSYRLV LPSLQLPVFH IPRTLFKFSL PDFKKLSTID NIYIPAMGNF TYDFSFKSSV ITLNTNAGLY NQSDLVARFL SSSSFVTDAL QYKLEGTSRL MRKKVLKLAT AVSLTNKFLK GSHDSTISLT KKNMEASVKT TANLHAPIFT MNFKQELNGN TKSKPTVSSS IELNYDFNSS KLHSAAKGGV DHKFSLESLT SYLSIESFTK GNIKGSFLSQ EYSGSVANEA NVYLNSKGTR SSVRLQGASN FAGIWNFEVG ENFAGEATLR RIYGTWEHNM INHLQVFSYF DTKGKQTCRA TLELSPWTMS TLLQVHVSQP SPLFDLHHFD QEVILKASTK NQKVSWKSEV QVESQVLQHN AHFSNDQEEV RLDIAGSLEG QLWDLENFFL PAFGKSLREL LQIDGKRQYL QASTSLHYTK NPNGYLLSLP VQELTDRFII PGLKLNDFSG IKIYKKLSTS PFALNLTMLP KVKFPGVDLL TQYSKPEGSS VPTFETTIPE IQLTVSQFTL PKSFPVGNTV FDLNKLTNLI ADVDLPSITL PEQTIEIPSL EFSVPAGIFI PFFGELTAHV GMASPLYNVT WSTGWKNKAD HVETFLDSTC SSTLQFLEYA LKVVGTHRIE NDKFIYKIKG TLQHCDFNVK YNEDGIFEGL WDLEGEAHLD ITSPALTDFH LHYKEDKTSV SASAASPAIG TVSLDASTDD QSVRLNVYFR PQSPPDNKLS IFKMEWRDKE SDGETYIKIN WEEEAAFRLL DSLKSNVPKA SEAVYDYVKK YHLGHASSEL RKSLQNDAEH AIRMVDEMNV NAQRVTRDTY QSLYKKMLAQ ESQSIPEKLK KMVLGSLVRI TQKYHMAVTW LMDSVIHFLK FNRVQFPGNA GTYTVDELYT IAMRETKKLL SQLFNGLGHL FSYVQDQVEK SRVINDITFK CPFSPTPCKL KDVLLIFRED LNILSNLGQQ DINFTTILSD FQSFLERLLD IIEEKIECLK NNESTCVPDH INMFFKTHIP FAFKSLRENI YSVFSEFNDF VQSILQEGSY KLQQVHQYMK AFREEYFDPS VVGWTVKYYE IEEKMVDLIK TLLAPLRDFY SEYSVTAADF ASKMSTQVEQ FVSRDIREYL SMLADINGKG REKVAELSIV VKERIKSWST AVAEITSDYL RQLHSKLQDF SDQLSGYYEK FVAESTRLID LSIQNYHMFL RYIAELLKKL QVATANNGLL KRGDFEAAVK LGIACLYNEG LSVSDEAYAE VNGLKASRFF SMDERLNMGS DPFIWLSICP PCFRKLRDFA GKGCWEAQPA LAKDCAGGSQ LGLEGKAFSE SVCQLFQASQ AVNKQQIFSV QKGLSDTVRY ILIGWLVEVA PMKDFTSLCL HLTVECVGRY LQRKLVPRYK LQLLGIACMV ICTWFISKEI LTIREAVRLT DNTYKYKDLV RVKREIISAL EGKIRIPTVV DYKEVLLTLV PVTPRTQYLC SFLCELTLSV YTPAHLASAA LLLARLMHGQ TQP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Gene References into Functions

enhanced VLDL TG secretion in the absence of hepatocyte ABCA1 is due to altered intracellular trafficking of apolipoprotein B (apoB), resulting in augmented TG addition to nascent VLDL. PMID:28694219
Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID:27495870
Coordinated up-regulation of Mttp, Apob, Hnf1a, Hnf4a, Fasn, Gpam and Dgat (1 and 2) gene expressions may contribute to the increase in circulating and liver concentrations of TAGs after lipectomy in an experimental rat model PMID:26184122
Overall, results further support the importance of Class I PI3K activity in IDAD, and suggest that loss of this activity is sufficient to cause resistance. PMID:26616056
KLHL12 plays a critical role in facilitating the endoplasmic reticulum exit and secretion of apoB100-VLDL particles, suggesting that KLHL12 modulation would influence plasma lipid levels. PMID:24334870
Report abnormal gene/protein expression and phosphorylation of multiple pathways related to inflammatory, insulin signaling and lipogenesis may be important underlying factors in VLDL-apoB100 particles overproduction observed in type 2 diabetes. PMID:22546076
Doxycycline induced apoA-IV expression in McA-RH7777 cells alters apoB and lipid secretion. PMID:22257482
JCR:LA-cp rats are used as a model of postprandial lipemia to explore chylomicron metabolism during the onset and development of insulin-resistance by showing impaired apolipoprotein b-48 blood levels. PMID:16624317
It was concluded that IL-6 favors secretion of apoB-containing lipoproteins by increasing availability of apoB through changes in apob gene transcription. PMID:20651008
A factor(s) other than microsomal triglyceride transfer protein mediates the formation of the phospholipid-rich primordial apoB:1000-containing initiation complex. PMID:20181985
the developmental regulation of apoB mRNA editing is an autonomous cytodifferentiation function of small intestine and involves Cdx1 PMID:12493769
pulse/chase protocols and subcellular fractionation showed apoB100 bulk lipidation, resulting in conversion of LDL/HDL to VLDL, can occur in the ER, but not in the Golgi, in McA RH7777 cells PMID:12917397
apoB100 lipoproteins exit the endoplasmic reticulum in COPII vesicles, but under conditions favorable for very low density lipoprotein formation final lipid loading occurs post-endoplasmic reticulum PMID:12960170
apoB mRNA editing is modulated at the level of assembly by alterations in the relative abundance of multiple related RNA-binding auxiliary proteins. PMID:14570923
increased FA delivery to the liver increases secretion of apoB; oleic acid -induced apoB secretion occurred via mechanisms other than by providing substrate for TG synthesis; and the route of delivery of FA is important for its effects on apoB secretion. PMID:14970200
Lipid peroxidation and oxidative stress regulate hepatic Apob degradation and VLDL production. PMID:15124019
the dense apoB-100 intracellular particle is converted to LDL-VLDL 2 by size-dependent lipidation PMID:15520448
Hepatic Betaine-Homocysteine S-Methyltransferase expression was induced by feeding rats a Met (L-methionine)-restricted betaine-containing diet, and parameters of ApoB metabolism were evaluated. PMID:16396637
apolipoprotein B secretion, but not lipid, is upregulated by tumor necrosis factor-alpha in rat hepatocyte cultures in the absence of extracellular fatty acids PMID:17405916
the initial addition of phospholipids to apoB:1000 and initiation of apoB-containing lipoprotein assembly occur independently of MTP lipid transfer activity. PMID:17690102
apoB secretory control via PERPP/autophagosomes is likely a key component of normal and pathologic regulation of plasma apoB levels, as well as a means for remarkably late-stage quality control of a secreted protein PMID:18391222
Localization of microsomal triglyceride transfer protein in the Golgi: possible role in the assembly of chylomicrons PMID:11830580