Recombinant Rat ADP-ribosylation factor 6 (Arf6)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Arf6

Uniprot NO.

P62332

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

2-175

Target Protein Sequence

GKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1. Required for NTRK1 sorting to the recycling pathway from early endosomes.

Gene References into Functions

both Arf6 activation through GluN2B-BRAG1 during early development and the transition from BRAG1- to BRAG2-dependent Arf6 signaling induced by the GluN2 subunit switch are critical for the development of mature glutamatergic synapses. PMID:26884337
The authors propose that Arf6 acts at the presynapse to define the fate of an endocytosed synaptic vesicle. PMID:26731518
Ang II promotes activation of ARF6 to controls reactive oxygen species production by regulating Rac1 activation and Nox1 expression. PMID:26824355
Developmental upregulation of Arf6 enhanced retrograde transport in CNS axons. PMID:26019348
blocking neuronal activity in developing neurons using tetrodotoxin or enhancing the activity in mature neurons using picrotoxin or chemical long term potentiation reversed the effect of ARF6 on each stage PMID:25605715
Rab35/ACAP2 and cytohesin-2 antagonistically control oligodendrocyte differentiation and myelination through Arf6 regulation. PMID:24600047
hERG undergoes clathrin-independent endocytosis via a mechanism involving Arf6 PMID:24392021
findings demonstrate an essential role of TBC1D24 in neuronal migration and maturation and highlight the physiological relevance of the ARF6-dependent membrane-trafficking pathway in brain development. PMID:24469796
Our data suggest a novel mechanism of integrin traffic and transport in peripheral axons, regulated by the activation state of ARF6 PMID:22836268
Rab35 regulates Arf6 activity through centaurin-beta2 (ACAP2) during neurite outgrowth. PMID:22344257
Studies indicate that ARF6 needs to be active to regulate the size of the blebs. PMID:19845506
Gas3/PMP22-induced vacuoles do not contain transferrin receptor, but instead they trap membrane proteins that normally traffic through the ADP-ribosylation factor 6 (Arf6) endosomal compartment. PMID:12584243
GIT1 represents part of the pathway that inactivates ARF6-dependent reactions and thereby negatively regulates and/or terminates exocytotic release PMID:16439353
endotubin labels a distinct endosomal structure in neurons; and ARNO and ARF6 mediate neurite extension through the regulation of this compartment PMID:17897316
ARF6 is a critical upstream signaling element in the activation of PLD necessary to produce the fusogenic lipids required for exocytosis. PMID:19124467
ATF6 is activated by ischemia but inactivated upon reperfusion, suggesting that it may play a role in the induction of ER stress response genes during ischemia that could have a preconditioning effect on cell survival during reperfusion. PMID:19622751
Arf6 is a key player in mammalian peroxisome biogenesis. PMID:19686593

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Subcellular Location

Cytoplasm, cytosol. Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Recycling endosome membrane; Lipid-anchor. Cell projection, filopodium membrane; Lipid-anchor. Cell projection, ruffle. Cleavage furrow. Midbody, Midbody ring. Early endosome membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor.

Protein Families

Small GTPase superfamily, Arf family

Database Links

UNIGENE: Rn.6993

KEGG: rno:79121

STRING: 10116.ENSRNOP00000006355

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