Recombinant Pig Osteopontin (SPP1)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SPP1

Uniprot NO.

P14287

Species

Sus scrofa (Pig)

Source

Yeast

Expression Region

17-303

Target Protein Sequence

LPVK QTNSGSSEEK LLSNKYTDAV ATLLKPDPSQ KQTFLAPQNT ISSEETDDFK QETLPSKSNE SPEQTDDVDD DDDEDHVDSR DTDSEEADHA DDADRSDESH HSDESDELVT DFPTDTPATD VTPAVPTGDP NDGRGDSVVY GLRSKSKKFR RSEAQQLDAT EEDLTSHVES EETDGTPKAI LVAQRLHVAS DLDSQEKDSQ ETSQPDDRSV ETRSQEQSKE YTIKTYDGSN EHSNVIESQE NPKVSQEFHS HEDKLVPDSK SEEDKHLKLR VSHELESASS EIN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Gene References into Functions

These data suggested that methylation in the OPN promoter plays a crucial role in the regulation of OPN expression that we found in cloned pigs genome. PMID:25101273
The striking breed differences between hyperprolific Large White and Meishanin pigs of secreted phospoprotein 1 expression in endometrium suggest that SPP1 may be associated with placental efficiency. PMID:23575146
Microglia incubated in vitro with different concentrations (0.1 fM-1 nM) of recombinant osteopontin showed increased proliferation at 10 fM. PMID:22974008
results of this study reveal faster growth rate and differences in pig productivity according to genotypes of the SPP1 gene PMID:22391653
Osteopontin could play an important role in the development of neointimal hyperplasia in venous conduits after coronary artery bypass grafting. PMID:22219474
A different MSSCP pattern was shown in osteopontin which indicates that mutation is located in promotor region; the A --> G transitions was identified in two positions -617 and -608 PMID:20010486
The 3' terminal end of the first intron of porcine SPP1 harbors a C/EBPbeta binding site and this binding site is negatively affected by the mutant G allele. PMID:19845957
in pregnant pigs SPP1 is induced by conceptus estrogen in uterine luminal epithelium and is regulated in a manner coincident with placental progesterone production PMID:16120824
osteopontin promotes pathologic mineralization via calcium pyrophosphate dihydrate crystal formation in articular cartilage. PMID:17123806
Osteopontin is detected in the majority of germ cells and is involved in spermatogenesis in boar testis. PMID:17519561