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Recombinant Pig Interferon regulatory factor 3 (IRF3)

Recombinant Pig Interferon regulatory factor 3 (IRF3)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

IRF3

Purity

>85% (SDS-PAGE)

Target Names

IRF3

Uniprot NO.

Q764M6

Alternative Names

IRF3Interferon regulatory factor 3; IRF-3

Species

Sus scrofa (Pig)

Source

Yeast

Expression Region

1-419

Target Protein Sequence

MGTQKPRILP WLISQLNQGQ LEGVAWLDEG HTRFRIPWKH GLRQDAQQED FGIFQAWAEA SGAYTPGKDK PDLPTWKRNF RSALNRKEAL RLAEDHSKDP HDPHKIYEFV TSGVGDFPEP DTSLDLSGRY STSDTHEDSL DKLLSGMDLA SDAGPQSLTL ALEQPPQLSL SPSVDAPASC PNLGVRENPL KQLLANDDEW EFQVTVFYRG CQVFQQTVCS PGGLRLVGSE AEDGTLAGQP VRLPDPAASL TDRGVADYVR RVLSCLGGGL ALWRAGQWLW AQRLGHCHVY WAMGEELIPD SGHKPDGEVP KDREGGVFDL GPFIEDLIAF IEGSRRSPRY TLWFCMGQSW PQDEPWVKRL VMVKVVPMCL RALVDMARDG GASSLENTVD LHISNSHPLS LTSDQYKACL RDLVEDMDF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Gene References into Functions

The authors used recombinant classical swine fever virus N(pro) and swine IRF3 proteins and show that N(pro) interacts with IRF3 directly without additional proteins and forms a soluble 1:1 complex. PMID:27334592
Amino acid residues in the N-terminal domain of Npro are involved in the stability of Npro, in interaction of Npro with IRF-3 and subsequent degradation of IRF-3, leading to downregulation of IFN-alpha/beta production. PMID:25809915
The obtained results showed that PRRSV nsp1 could inhibit Poly(I:C)-induced IFN-beta promoter activity in MARC-145 cells by down-regulating the protein level of IRF-3 and inhibiting the phosphorylation of IRF-3. PMID:20692306
proteasomal degradation of IRF3 is induced by a direct or indirect interaction with N(pro). PMID:17215286