Recombinant Pig Hormone-sensitive lipase (LIPE), partial

Product Details

Abbreviation
LIPE
Purity
>85% (SDS-PAGE)
Target Names
LIPE
Uniprot NO.
Species
Sus scrofa (Pig)
Source
Yeast
Expression Region
1-764
Target Protein Sequence
MDLRTMTQSL VTLAEDNMAF FSGQGPGETA RRLSGVFAGI REQALGLEPA LGRLLSVAHL FDLDAETPAN GYRSLVHTAR CCLAHLLHKS RYVASNRRSI FFRTSHNLAE LEAYLAALTQ LRALAYYAQR LLAINRPGKL FFEGDEGITA DFLREYVTLH KGCFYGRCLG FQFTPAIRPF LQTISIGLVS FGEHYKRNET GLSVTASSLF TSGRFAIDPE LRGAEFERII QNLDVHFWKA FWNITEIEVL SSLANMASAT VRVSRLLSLP PKAFEMPLTA DPKLTVTISP PLAHTGPGPV LVRLISYDLR EGQDSEELSS LVRSEGPRGL ELRPRPQQAP RSRSLVVHIH GGGFVAQTSK SHEPYLKSWA QELGVPILSI DYSLAPEAPF PRALEECFYA YCWAVKHCGL LGSTGERICL AGDSAGGNLC FTVSLRAAAY GVRVPDGIMA AYPATMLQSA ASPSRLLSLM DPLLPLSVLS KCVSAYAGGE MEDHSDSDQK ALGMMGLVRR DTALLFRDLR LGASSWLNSF LELSGHKSRP NLVPTEEPMR RSVSEAALAQ PEGPLGTDSL KYLTLHDLSL SSETQDTPEL SLSAETLGPT TPSAVNFLFR PEDAPEEAEA RDDISTKEEK VYSVRAAFPE GFHPRRSSQG AIQMPLYSAP IVKNPFMSPL LAPDSMLQTL PPVHIVACAL DPMLDDSVMF ARRLRSLGQP VTLHVVEDLP HGFLSLAALC RETRQAAALC VDRIRFILNP PGPATPAGPT TPPV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial
Tag Info
N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs. Preferentially hydrolyzes fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs and FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Subcellular Location
Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Lipid droplet.
Protein Families
'GDXG' lipolytic enzyme family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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