Recombinant Pig Cellular tumor antigen p53 (TP53)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

TP53

Purity

>85% (SDS-PAGE)

Target Names

TP53

Uniprot NO.

Q9TUB2

Species

Sus scrofa (Pig)

Source

Yeast

Expression Region

1-386

Target Protein Sequence

MEESQSELGV EPPLSQETFS DLWKLLPENN LLSSELSLAA VNDLLLSPVT NWLDENPDDA SRVPAPPAAT APAPAAPAPA TSWPLSSFVP SQKTYPGSYD FRLGFLHSGT AKSVTCTYSP ALNKLFCQLA KTCPVQLWVS SPPPPGTRVR AMAIYKKSEY MTEVVRRCPH HERSSDYSDG LAPPQHLIRV EGNLRAEYLD DRNTFRHSVV VPYEPPEVGS DCTTIHYNFM CNSSCMGGMN RRPILTIITL EDASGNLLGR NSFEVRVCAC PGRDRRTEEE NFLKKGQSCP EPPPGSTKRA LPTSTSSSPV QKKKPLDGEY FTLQIRGRER FEMFRELNDA LELKDAQTAR ESGENRAHSS HLKSKKGQSP SRHKKPMFKR EGPDSD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.

Gene References into Functions

Inhibition of p53 signaling pathway can reverse the cell cycle arrest in G0/G1 phase induced by Porcine epidemic diarrhea virus infection. PMID:29800601
in a pig model of human familial adenomatous polyposis, p53 plays a role in the early precancerous stages of polyp development PMID:30145695
Knockdown of p53 led to three outcomes: 1) cell death was attenuated; 2) Tunicamycin-induced redistribution of GRP78 from the nucleus to the endoplasmic reticulum lumen was recovered; and 3) the endoplasmic reticulum-fluorescence/EGFP-calreticulin was recovered. PMID:30121649
This study demonstrated that various expression levels of p53 in porcine fibroblasts could be achieved by gene targeting and RNA interference. PMID:27142766
SIRT1, p53 and NF-kappaB are involved in the control of both the proliferation and the apoptosis of ovarian cells. PMID:25027053
Taken together,these results demonstrated that Porcine parvovirus infection induced apoptosis in PK-15 cells through activation of p53 and mitochondria-mediated apoptosis pathway. PMID:25499817
Data indicate that higher hepatic glucocorticoid receptor (GR) expression in EHL piglets attributes mainly to the enhanced transcription of GR promoter 1-9/10 from breed-specific interaction of p53 and specificity protein 1 (Sp1). PMID:23950944
Taken together, these results demonstrated that transmissible gastroenteritis virus infection promoted the activation of p38 MAPK and p53 signalling, and p53 signalling might play a dominant role in the regulation of cell apoptosis. PMID:23677787
role of transcription factor p53 and the metabolic hormone leptin, in controlling basic functions (proliferation, apoptosis and secretory activity) of ovarian cells PMID:22151798
differential expression of p53 pathway-related genes accounts for breed-specific skeletal muscle and meat characteristics PMID:19937136
CONCLUSION(S): (1) Apoptosis is involved in follicular atresia; (2) Bcl-2 is induced by warm ischemia; and (3) cryopreservation insult does not alter the apoptotic signals with short tissue preparation time. PMID:16616078
Suppressive effect of FSH and LH on p53 expression and caspase-3 activity with parallel increase in bcl-2 expression and increased P production was observed. PMID:17437340
p53 signaling might be a major determinant for the replicative senescence in the miniature pig fibroblast cells that have the shorter lifespan and slower growth rate compared to primary domestic pig fibroblast cells in vitro. PMID:17543094
exogenous in vivo NO treatment seems to preserve VSMC from mitochondrial-dependent apoptosis and drive cells to quiescence through p53 increase PMID:19124124

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Families

P53 family

Database Links

UNIGENE: Ssc.15917

KEGG: ssc:397276

STRING: 9823.ENSSSCP00000019016

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