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Recombinant Oryza sativa subsp. japonica Quercetin 3-O-methyltransferase 1 (ROMT-9)

Recombinant Oryza sativa subsp. japonica Quercetin 3-O-methyltransferase 1 (ROMT-9)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

ROMT-9

Purity

>85% (SDS-PAGE)

Target Names

COMT

Uniprot NO.

Q6ZD89

Alternative Names

COMT; ROMT-9; Os08g0157500; LOC_Os08g06100; OsJ_26105; P0438H08.29; Flavone 3'-O-methyltransferase 1; OsOMT1; EC 2.1.1.42; Acetylserotonin O-methyltransferase COMT; EC 2.1.1.4; Caffeate O-methyltransferase 1; OsCOMT1; EC 2.1.1.68; OsCOMT; Quercetin 3'-O-methyltransferase 1

Species

Oryza sativa subsp. japonica (Rice)

Source

Yeast

Expression Region

3-368

Target Protein Sequence

STAADMAA AADEEACMYA LQLASSSILP MTLKNAIELG LLETLQSAAV AGGGGKAALL TPAEVADKLP SKANPAAADM VDRMLRLLAS YNVVRCEMEE GADGKLSRRY AAAPVCKWLT PNEDGVSMAA LALMNQDKVL MESWYYLKDA VLDGGIPFNK AYGMTAFEYH GTDARFNRVF NEGMKNHSVI ITKKLLDLYT GFDAASTVVD VGGGVGATVA AVVSRHPHIR GINYDLPHVI SEAPPFPGVE HVGGDMFASV PRGGDAILMK WILHDWSDEH CARLLKNCYD ALPEHGKVVV VECVLPESSD ATAREQGVFH VDMIMLAHNP GGKERYEREF RELARAAGFT GFKATYIYAN AWAIEFTK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Methylates OH residues of flavonoid compounds. Can methylate eriodictyol, luteolin, quercetin and taxifolin. Methylates caffeate to produce ferrulate. Catalyzes the methylation of monolignols, the lignin precursors. Functions cooperatively with CAD2 in the culm internodes for the biosynthesis of monolignols. May be involved in lignin biosynthesis in leaves and roots. Involved in syringyl lignin biosynthesis. Can function as 5-hydroxyconiferaldehyde O-methyltransferase in the biosynthetic pathway to syringyl lignin (Ref.16). Involved in melatonin biosynthesis. Can function as acetylserotonin O-methyltransferase. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).

Subcellular Location

Cytoplasm.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family, COMT subfamily

Tissue Specificity

Expressed in roots and stems, and at lower levels in leaves.

Database Links

UNIGENE: Os.11202

KEGG: osa:4344702

STRING: 39947.LOC_Os08g06100.1

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