Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB)

CSB-EP363658MVZ
Size:
20μg
20μg100μg1mg(1mg*1 or 500ug*2)
US$388
Quantity:
Express system: E.coli
Species: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Tag Info: N-terminal 6xHis-tagged
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
esxB
Uniprot NO.
Research Area
Others
Species
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Source
E.coli
Expression Region
2-100aa
Target Protein Sequence
AEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF
Mol. Weight
14.7
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Buffer
Tris-based buffer,50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Troubleshooting and FAQs
Datasheet & COA
Please contact us to get it.
Images
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description

This recombinant M.tb esxB protein is an E.coli expressed protein (Full Length of Mature Protein) with N-terminal 6xHis tag and its purity is 90%+ determined by SDS-PAGE. With the appropriate cDNA and PCR methods, esxB expression plasmids can be rapidly produced. which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, esxB proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, esxB proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.

esxB, also called CFP-10, is a Mycobacterium tuberculosis secreted antigen that contributes to viral virulence and pathogenicity. CFP-10 and ESAT-6 forms a 1:1 heterodimeric complex that plays a critical role in the pathogenesis of tuberculosis (TB). Amanda Welin et al. has shown that the ESAT-6/CFP-10 complex induces a transient release of Ca2+ from intracellular stores in human neutrophils. CFP-10 rather than ESAT-6 was responsible for triggering the Ca2+ response, in a pertussis toxin-sensitive manner, implying the participation of a GPCR.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function
A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.
Subcellular Location
Secreted.
Protein Families
WXG100 family, CFP-10 subfamily
Database Links

KEGG: mtc:MT3988

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