Abbreviation
Recombinant Mouse Plau protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Fully active measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Plau needs to be activated by Plasmin to be enzymatically active. The specific activity is above 2000 pmol/min/ug.
Alternative Names
Urokinase-type plasminogen activator; U-plasminogen activator; uPA; EC 3.4.21.73 [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B]
Species
Mus musculus (Mouse)
Expression Region
21-433aa
Target Protein Sequence
GSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Fully active measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Plau needs to be activated by Plasmin to be enzymatically active. The specific activity is above 2000 pmol/min/ug.
Description
Urokinase-type plasminogen activator plays a central role in extracellular matrix remodeling and cancer cell invasion, making its enzymatic characterization essential for understanding tumor progression mechanisms. This recombinant mouse Plau, spanning the full mature protein sequence (aa 21–433), demonstrates robust catalytic function with a specific activity exceeding 2000 pmol/min/μg against the fluorogenic substrate Z-GGR-AMC following plasmin activation, providing quantitative performance data that supports kinetic parameter analysis (Km, Vmax, kcat/Km) and inhibitor screening with reliable IC50 determination. The C-terminal 10×His tag preserves access to the catalytic domain while enabling straightforward purification, and the >95% purity by SDS-PAGE meets the quality thresholds commonly required for accurate enzymatic assays and structural studies where contaminants would confound kinetic measurements or crystallization trials. With endotoxin levels below 1.0 EU/μg, this preparation is appropriate for drug candidate evaluation and serves as a validated positive control in enzyme-linked assays investigating plasminogen activation pathways.
