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Recombinant Mouse Tyrosine-protein kinase HCK (Hck)

Recombinant Mouse Tyrosine-protein kinase HCK (Hck)

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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Hck

Uniprot NO.

P08103

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

2-524

Target Protein Sequence

GGRSSCEDP GCPRSEGRAP RMGCVKSRFL RDGSKASKTE PSANQKGPVY VPDPTSSSKL GPNNSNSMPP GFVEGSEDTI VVALYDYEAI HREDLSFQKG DQMVVLEEAG EWWKARSLAT KKEGYIPSNY VARVNSLETE EWFFKGISRK DAERHLLAPG NMLGSFMIRD SETTKGSYSL SVRDFDPQHG DTVKHYKIRT LDSGGFYISP RSTFSSLQEL VLHYKKGKDG LCQKLSVPCV SPKPQKPWEK DAWEIPRESL QMEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF LAEANLMKSL QHDKLVKLHA VVSQEPIFIV TEFMAKGSLL DFLKSEEGSK QPLPKLIDFS AQISEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK WTAPEAINFG SFTIKSDVWS FGILLMEIVT YGRIPYPGMS NPEVIRALEH GYRMPRPDNC PEELYNIMIR CWKNRPEERP TFEYIQSVLD DFYTATESQY QQQP

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Gene References into Functions

Hematopoietic Hck promotes tumorigenesis. PMID:28399411
Data suggest that Hck is a key mediator of renal fibrosis PMID:27927780
Hck is not only progressively overexpressed in atherosclerosis, but it also controls critical molecular processes in monocyte influx, blood monocyte subset balance, macrophage accumulation, and the maintenance of atherosclerotic lesion stability. PMID:26068045
tyrosine phosphorylation of WASP by Hck is required for proper macrophage functions PMID:24482227
Hck is one of the very few effectors of preosteoclast recruitment described to date and thereby plays a critical role in bone remodeling. PMID:23742809
Despite possessing enhanced killing, alveolar macrophage Hck/Fgr/Lyn-deficient ("triple-knockout") mice do not demonstrate enhanced inflammatory responses to Pneumocystis murina. PMID:21220696
Taken together, PKR and Hck were critical for DON-induced ribosomal recruitment of p38, its subsequent phosphorylation, and, ultimately, p38-driven proinflammatory cytokine expression. PMID:20181660
Nef participates in HIV-1-induced multinucleated giant cells formation via a p61Hck- and lysosomal enzyme-dependent pathway PMID:20488787
spatial organization of podosomes as large rosettes, proteolytic degradation of extracellular matrix, and 3D migration appeared to be functionally linked and regulated by Hck in macrophages PMID:19897576
enhanced innate immune response in Hck(F/F) mice thereby skewing innate immunity from a reversible physiological host defense response to one causing irreversible tissue damage PMID:12208875
BCR/ABL SH3 and SH2 domains interact with hematopoietic cell kinase (Hck), leading to the stimulation of Hck catalytic activity PMID:12411494
Hck plays no role in chemoattractant-induced, inside-out beta 2 integrin activation of neutrophils but regulates their outside-in, signaling-dependent sustained adhesion. PMID:16785558
Lyn activity is elevated in hck(-/-) cells, leading to increased phosphorylation of several negative regulators PMID:17513616
a myeloproliferative transformation-sensitive signaling pathway, composed of Lyn/Hck, SHIP, autocrine/paracrine cytokines, and Stat5, that regulates HSC differentiation and M2 macrophage programming. PMID:18246197
Alternate protein isoforms arise through the use of a non-AUG (CUG), and a downstream in-frame AUG, translation initiation codons, in mouse and human. PMID:1875927

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Subcellular Location

Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.; [Isoform 1]: Membrane; Lipid-anchor. Membrane, caveola. Lysosome. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol.; [Isoform 2]: Cell membrane; Lipid-anchor. Membrane, caveola; Lipid-anchor. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, SRC subfamily

Tissue Specificity

Expressed predominantly in cells of the myeloid and B-lymphoid lineages.

Database Links

UNIGENE: Mm.715

KEGG: mmu:15162

STRING: 10090.ENSMUSP00000003370

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