Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Tert; Telomerase reverse transcriptase; EC 2.7.7.49; Telomerase catalytic subunit
Species
Mus musculus (Mouse)
Expression Region
595-928aa
Target Protein Sequence
EVRHHQDTWLAMPICRLRFIPKPNGLRPIVNMSYSMGTRALGRRKQAQHFTQRLKTLFSMLNYERTKHPHLMGSSVLGMNDIYRTWRAFVLRVRALDQTPRMYFVKADVTGAYDAIPQGKLVEVVANMIRHSESTYCIRQYAVVRRDSQGQVHKSFRRQVTTLSDLQPYMGQFLKHLQDSDASALRNSVVIEQSISMNESSSSLFDFFLHFLRHSVVKIGDRCYTQCQGIPQGSSLSTLLCSLCFGDMENKLFAEVQRDGLLLRFVDDFLLVTPHLDQAKTFLSTLVHGVPEYGCMINLQKTVVNFPVEPGTLGGAAPYQLPAHCLFPWCGLLL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 6xHis-SUMO-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
Amino acids 595-928 form the expressed segment for recombinant Mouse Tert. The theoretical molecular weight of the Tert protein is 54.1 kDa. This Tert recombinant protein is manufactured in e.coli. The Tert coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant Tert protein in following stages of expression and purification.
The telomerase reverse transcriptase (TERT) is a crucial enzyme involved in maintaining telomere length, which is essential for chromosomal stability and cellular lifespan. TERT is a catalytic subunit of the telomerase complex, responsible for adding telomeric DNA repeats to the ends of chromosomes during DNA replication. This process counteracts the gradual shortening of telomeres that occurs with each cell division. In mice, TERT plays a fundamental role in preventing premature cellular senescence and supporting tissue homeostasis. The enzyme is particularly active in stem cells and highly proliferative cells, contributing to their ability to undergo numerous cell divisions. Research areas related to TERT include aging, cancer, and regenerative medicine, as its activity is tightly linked to cellular processes influencing both normal development and pathological conditions. Understanding the regulation and function of TERT holds significant implications for potential therapeutic strategies targeting age-related diseases and cancer.
