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Recombinant Mouse Serine/threonine-protein kinase PLK4 (Plk4), partial

Recombinant Mouse Serine/threonine-protein kinase PLK4 (Plk4), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Plk4

Purity

>85% (SDS-PAGE)

Target Names

Plk4

Uniprot NO.

Q64702

Alternative Names

Plk4; Sak; Stk18; Serine/threonine-protein kinase PLK4; EC 2.7.11.21; Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase Sak

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-925

Target Protein Sequence

MAACIGERIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE VKIHCQLKHP SVLELYNYFE DNNYVYLVLE MCHNGEMNRY LKNRMKPFSE REARHFMHQI ITGMLYLHSH GILHRDLTLS NILLTRNMNI KIADFGLATQ LNMPHEKHYT LCGTPNYISP EIATRSAHGL ESDIWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PAFLSREAQD LIHQLLRRNP ADRLSLSSVL DHPFMSRNPS PKSKDVGTVE DSMDSGHATL STTITASSGT SLSGSLLDRR LLVGQPLPNK ITVFQKNKNS SDFSSGDGSN FCTQWGNPEQ EANSRGRGRV IEDAEERPHS RYLRRAHSSD RASPSNQSRA KTYSVERCHS VEMLSKPRRS LDENQHSSNH HCLGKTPFPF ADQTPQMEMV QQWFGNLQMN AHLGETNEHH TVSPNRDFQD YPDLQDTLRN AWTDTRASKN ADTSANVHAV KQLSAMKYMS AHHHKPEVMP QEPGLHPHSE QSKNRSMEST LGYQKPTLRS ITSPLIAHRL KPIRQKTKKA VVSILDSEEV CVELLRECAS EGYVKEVLQI SSDGTMITVY YPNDGRGFPL ADRPPLPTDN ISRYSFDNLP EKYWRKYQYA SRFIQLVRSK TPKITYFTRY AKCILMENSP GADFEVWFYD GAKIHKTENL IHIIEKTGIS YNLKNENEVT SLKEEVKVYM DHANEGHRIC LSLESVISEE EKRSRGSSFF PIIVGRKPGN TSSPKALSAP PVDPSCCKGE QASASRLSVN SAAFPTQSPG LSPSTVTVEG LGHTATATGT GVSSSLPKSA QLLKSVFVKN VGWATQLTSG AVWVQFNDGS QLVVQAGVSS ISYTSPDGQT TRYGENEKLP EYIKQKLQCL SSILLMFSNP TPNFQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification. Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites.

Gene References into Functions

Aurora A and Plk4 are rate-limiting factors contributing to microtubule growth as the acentriolar oocyte resumes meiosis. PMID:28972102
that aneuploidy induced by transient centrosome amplification can accelerate tumorigenesis in p53-deficient cells PMID:26595384
Transient knockdown of KLF14 is sufficient to induce Plk4-directed centrosome amplification. PMID:26439168
PLK4 is essential for meiotic resumption but may not influence spindle formation in mouse oocytes during meiotic maturation PMID:25740542
p53-Dependent and cell specific epigenetic regulation of the polo-like kinases under oxidative stress. PMID:24498222
The Plk4-Cep152 complex has an unexpected role in promoting microtubule nucleation in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles. PMID:24268700
Loss of Plk4 is associated with centrosome amplification causing microcephaly. PMID:23666084
PP2A (Protein Phosphatase 2A(Twins)) counteracts Plk4 autophosphorylation, thus stabilizing Plk4 and promoting centriole duplication PMID:21987638
the I242N heterozygous mutation in PLK4 is causative for patchy germ cell loss beginning at P10, suggesting a role for PLK4 during the initiation of spermatogenesis. PMID:21791561
Aberrant Plk methylation is correlated with the development of hepatocellular carcinoma in mice. PMID:21324136
Plk4 is required for cytokinesis and maintenance of chromosomal stability PMID:20348415
We propose that kinase-mediated, autoregulated instability of Plk4 self-limits Plk4 activity so as to prevent centrosome amplification. PMID:20100909
2.0 A crystal structure of a novel domain composed of the polo box motif of murine Sak PMID:12352953
polo-like kinase Plk4 (also called Sak) is required for late mitotic progression, cell survival and postgastrulation embryonic development PMID:16025114
Study shows that polo-like kinase Plk4 mediate the nucleolar release of Hand1 for its dimerization and biological function. PMID:17891141
Plk4 haploinsufficiency leads to changes in the levels of RNA accumulation for a number of key cellular genes as well as changes in protein levels for several important cell cycle/DNA damage proteins PMID:19607708

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus, nucleolus. Cleavage furrow. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrioles throughout the cell cycle. According to PubMed:11301255, it localizes to the nucleolus during G2, to the centrosomes in G2/M, and to the cleavage furrow during cytokinesis. Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily

Tissue Specificity

expressed in tissues associated with mitotic and meiotic cell division. Highly expressed in testis.

Database Links

UNIGENE: Mm.3794

KEGG: mmu:20873

STRING: 10090.ENSMUSP00000026858

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