Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Signal Transduction
Species
Mus musculus (Mouse)
Expression Region
27-434aa
Target Protein Sequence
ASASASGSGPASELGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLPVYNKAAWKHYKANHEADDWCVPSREPKDMTTFRSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
The expression region of this recombinant Mouse Pdk1 covers amino acids 27-434. The theoretical molecular weight of the Pdk1 protein is 48.7 kDa. This Pdk1 recombinant protein is manufactured in baculovirus. The N-terminal 10xHis tag was fused into the coding gene segment of Pdk1, making it easier to detect and purify the Pdk1 recombinant protein in the later stages of expression and purification.
Pyruvate dehydrogenase kinase isoform 1 (Pdk1) is a highly studied protein, with a primary focus on metabolic regulation and the field of tumor biology. As a critical regulator of the tricarboxylic acid cycle, Pdk1 influences cellular energy metabolism by inhibiting decarboxylase activity through phosphorylation. One of its most crucial research directions is its role in tumor metabolism regulation. Pdk1 is found to be overexpressed in various cancers, closely linked to tumor growth and metabolic reprogramming. Additionally, Pdk1 is implicated in multiple research areas, including cardiovascular diseases, as its regulation of metabolic pathways and signaling networks positions it as a potential drug target for the treatment and prevention of related diseases.
