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Recombinant Mouse Probable ATP-dependent RNA helicase DDX4 (Ddx4)

Recombinant Mouse Probable ATP-dependent RNA helicase DDX4 (Ddx4)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

Ddx4

Purity

>85% (SDS-PAGE)

Target Names

Ddx4

Uniprot NO.

Q61496

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-702

Target Protein Sequence

MGDEDWEAEI LKPHVSSYVP VFEKDKYSSG ANGDTFNRTS ASSDIGESSK KENTSTTGGF GRGKGFGNRG FLNNKFEEGD SSGFWKESNN DCEDNQTRSR GFSKRGGCQD GNDSEASGPF RRGGRGSFRG CRGGFGLGRP NSESDQDQGT QRGGGLFGSR KPAASDSGNG DTYQSRSGSG RGGYKGLNEE VVTGSGKNSW KSETEGGESS DSQGPKVTYI PPPPPEDEDS IFAHYQTGIN FDKYDTILVE VSGHDAPPAI LTFEEANLCQ TLNNNIAKAG YTKLTPVQKY SIPIVLAGRD LMACAQTGSG KTAAFLLPIL AHMMRDGITA SRFKELQEPE CIIVAPTREL INQIYLEARK FSFGTCVRAV VIYGGTQFGH SVRQIVQGCN ILCATPGRLM DIIGKEKIGL KQVKYLVLDE ADRMLDMGFG PEMKKLISCP GMPSKEQRQT LLFSATFPEE IQRLAGDFLK SSYLFVAVGQ VGGACRDVQQ TILQVGQYSK REKLVEILRN IGDERTMVFV ETKKKADFIA TFLCQEKIST TSIHGDREQR EREQALGDFR CGKCPVLVAT SVAARGLDIE NVQHVINFDL PSTIDEYVHR IGRTGRCGNT GRAISFFDTD SDNHLAQPLV KVLSDAQQDV PAWLEEIAFS TYVPPSFSSS TRGGAVFASV DTRKNYQGKH TLNTAGISSS QAPNPVDDES WD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.

Gene References into Functions

BHR also decreased the mRNA level of MVH in the bone marrow, and increased mRNA and protein level of MVH in the ovary o fpremature ovarian failure mice PMID:27465335
Hgher expression levels of ~55-60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. PMID:27460133
In mouse oocytes the mitochondrion-originated germinal body-like structures accumulate MVH protein. PMID:24780413
The results indicate the presence of a developmentally regulated mechanism in which Hat1 and p46 control the physiology of the chromatoid body by acetylation of MVH. PMID:22223882
Vasa/Ddx4 gene can be involved in regulation of ground and primed states of pluripotency PMID:22288104
SPSB1 and SPSB4 bind strongly to both Par-4 and VASA peptides. PMID:20561531
MVH plays crucial roles in the early phase of the ping-pong amplification cycle PMID:20439430
The RNA helicase Vasa is another essential protein in germline development and arginine methylation is conserved across phyla PMID:20080973
Data show that RanBPM and mouse vasa homolog (Mvh) are closely associated with perinuclear RNA-protein complexes and chromatoid bodies, and suggests a functional relationship between translational regulation and microtubule nucleation during meiosis. PMID:14648869