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Recombinant Mouse Mediator of RNA polymerase II transcription subunit 12 (Med12), partial

Recombinant Mouse Mediator of RNA polymerase II transcription subunit 12 (Med12), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Med12

Uniprot NO.

A2AGH6

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-2190

Target Protein Sequence

MAAFGILSYE HRPLKRLRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA VSGDEHGSAK NVNFNPAKIS SNFSSIIAEK LRCNTLSDTG RRKSLMNQKD NFWLVTARSQ SAINTWFTDL AGTKPLTHLA KKVPIFSKKE EVFGYLAKYT VPVMRAAWLI KMTCAYYAAM SETKVKKKNT ADPFTEWTQI ITKYLWEQLQ KMAEYYRPGP AGSGGCGSTI GPLPHDVEMA IRQWDYNEKL ALFMFQDGML DRHEFLTWVL ECFEKIRPGE DELLKLLLPL LLRYSGEFVQ SAYLSRRLAY FCTRRLALQL DGVSSHSSHV IAAQSTSSLP TTPAPQPPTS STPSTPFSDL LMCPQHRPLV FGLSCILQTI LLCCPSALVW HYSLTDSRIK TGSPLDHLPI APSNLPMPEG NSAFTQQVRA KLREIEQQIK ERGQAVEVRW SFDKCQEATA GFTIGRVLHT LEVLDSHSFE RSDFSNSLDS LCNRIFGLGP SKDGHEISSD DDAVVSLLCE WAVSCKRSGR HRAMVVAKLL EKRQAEIEAE RCGESEAADE KGSVASGSLS APSAPIFQDV LLQFLDTQAP MLTDPRSESE RVEFFNLVLL FCELIRHDVF SHNMYTCTLI SRGDLAFGAP GPRPPSPFDD PTDDPERKEA EGSSSSKLED PGLSESMDID PSSSVLFEDM EKPDFSLFSP TMPCEGKGSP SPEKPDVEKE VKPPAKEKIE GTLGILYDQP RHVQYATHFP IPQEESCSHE CNQRLVVLFG VGKQRDDARH AIKKITKDIL KVLNRKGTAE TDQLAPIVPL NPGDLTFLGG EDGQKRRRNR PEAFPTAEDI FAKFQHLSHY DQHQVTAQVS RNVLEQITSF ALGMSYHLPL VQHVQFIFDL MEYSLSISGL IDFAIQLLNE LSVVEAELLL KSSDLVGSYT TSLCLCIVAV LRHYHACLIL NQDQMAQVFE GLCGVVKHGM NRSDGSSAER CILAYLYDLY TSCSHLKSKF GELFSDFCSK VKNTIYCNVE PSESNMRWAP EFMIDTLENP AAHTFTYTGL GKSLSENPAN RYSFVCNALM HVCVGHHDPD RVNDIAILCA ELTGYCKSLS AEWLGVLKAL CCSSNNGTCG FNDLLCNVDV SDLSFHDSLA TFVAILIARQ CLLLEDLIRC AAIPSLLNAA CSEQDSEPGA RLTCRILLHL FKTPQLNPCQ SDGNKPTVGI RSSCDRHLLA ASQNRIVDGA VFAVLKAVFV LGDAELKGSG FTVPGGTEEL PEEEGGGGSS GRRQGGRNIS VETASLDVYA KYVLRSICQQ EWVGERCLKS LCEDSNDLQD PVLSSAQAQR LMQLICYPHR LLDNEDGENP QRQRIKRILK NLDQWTMRQS SLELQLMIKQ TPNTEMNSLL ENIAKATIEV FQQSAETGSS SGSTASNMPS SSKTKPVLSS LERSGVWLVA PLIAKLPTSV QGHVLKAAGE ELEKGQHLGS SSRKERDRQK QKSMSLLSQQ PFLSLVLTCL KGQDEQREGL LASLHSQVHQ IVINWRENQY LDDCKPKQLM HEALKLRLNL VGGMFDTVQR STQQTTEWAQ LLLEIIISGT VDMQSNNELF TTVLDMLSVL INGTLAADMS SISQGSMEEN KRAYMNLVKK LQKDLGERQS DSLEKVHQLL PLPKQNRDVI TCEPQGSLID TKGNKIAGFD SIFKKEGLQV STKQKISPWE LFEGLKPSTA PLSWAWFGTV RVDRRVARGE EQQRLLLYHT HLRPRPRAYY LEPLPLPPED EEPPAPALLE PEKKAPEPPK TDKPGAAPPS TEERKKKSTK GKKRSQPATK NEDYGMGPGR SGPYGVTVPP DLLHHANPGS ISHLSYRQSS MGLYTQNQPL PAGGPRVDPY RPVRLPMQKL PTRPTYPGVL PTTMSTVMGL EPSSYKTSVY RQQQPTVPQG QRLRQQLQAK IQSQGMLGQS SVHQMTPSSS YGLQTSQGYT SYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS THPSTNPTLV DPTRHLQQRP SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MMGTMTPLSA QGVQAGVRST SILPEQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYH IRQQQQQQQM LRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQP HQQQQQAAPP QPQPQSQPQF QRQGLQQTQQ QQQTAALVRQ LQQQLSNTQP QPSTNIFGRY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.

Gene References into Functions

Study provide evidence that Med12 regulates neural stem cell gene expression programs linked to adhesion and cell cycle progression but suppresses its adhesion by repressing the expression of cell adhesion molecules. PMID:27188461
alterations in MED12-dependent enhancer regulation may control both physiological and malignant hematopoiesis. PMID:27570068
The data reveal how the interplay between PRC1, ncRNA and Mediator complexes controls pluripotency and cellular differentiation. PMID:27096886
Med1 and Med12, two subunits of the mediator complex implicated in transcription initiation and long-range enhancer/promoter loop formation, are dynamically recruited to the IgH locus enhancers. PMID:26903242
leiomyomatous, Med12 c.131G>A variant-expressing uteri developed chromosomal rearrangements. PMID:26193636
this work points to Med12 being an essential coregulator of transcription factors controlling neural tube closure. PMID:20589884
show that in Med12 hypomorphic embryos, the Wnt/planar cell polarity pathway is disrupted and that canonical Wnt/beta-catenin signaling is impaired. PMID:20630950
Nanog and Med12 function in concert to regulate Nanog target genes and identify a novel role for Med12 in embryonic stem cell regulation. PMID:19036726