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Recombinant Mouse M-phase inducer phosphatase 2 (Cdc25b)

Recombinant Mouse M-phase inducer phosphatase 2 (Cdc25b)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cdc25b

Uniprot NO.

P30306

Alternative Names

Cdc25b; Cdc25m2; M-phase inducer phosphatase 2; EC 3.1.3.48; Dual specificity phosphatase Cdc25B

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-576

Target Protein Sequence

MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS PVTTLTQTMH NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE SSESSDAGLC MDSPSPVDPQ MAERTFEQAI QAASRVIQNE QFTIKRFRSL PVRLLEHSPV LQSITNSRAL DSWRKTEAGY RAAANSPGED KENDGYIFKM PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW KMEVEELSPV AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK SVTPLEEQQL EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT VDGKHQDLKY ISPETMVALL TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA VNLPLERDAE TFLLQRPIMP CSLDKRIILI FHCEFSSERG PRMCRFIRER DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP MNHEAFRDEL RNFRLKTRSW AGERSRRELC SRLQDQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity.

Gene References into Functions

data suggest an important role of CDC25B for microtubule nucleation and organization. N-terminal of CDC25B is required for regulating the microtubule dynamics and mitotic function. PMID:27885657
LSD1 is essential for oocyte meiotic progression by upregulating CDC25B expression. PMID:26626423
The role of Cdc25c and Cdc25b in activating G2/M cell cycle checkpoint in zygote. PMID:25012723
AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis. PMID:25349079
Data indicate that 14-3-3epsilon is required for the mitotic entry in the fertilized mouse eggs and responsible for sequestering the CDC25B in cytoplasm. Its binding to CDC25B-S321 phosphorylated by PKA induces mitotic arrest. PMID:25059436
contributes to metaphase arrest in mouse oocytes PMID:23661366
Ser321 of Cdc25B is the specific binding site for 14-3-3epsilon binding. PMID:23326474
Protein kinase A the early development of mouse embryos by phosphorylation of S149 and S321 of CDC25B, which plays an important role in the regulation of G(2)/M transition in the mitotic cell cycle of fertilized mouse eggs. PMID:22348958
Cdc25B overexpression in early mouse two-cell embryos reverses two-cell block and promotes their development into four-cell stage by activating MPF. PMID:21141569
MCPH1, through its function in the Chk1-Cdc25-Cdk1 pathway to couple the centrosome cycle with mitosis, is required for precise mitotic spindle orientation and thereby regulates the progenitor division mode to maintain brain size. PMID:21947081
Ser(149) may be another potential PKA phosphorylation target of Cdc25B in G(2)/M transition of fertilized mouse eggs and Cdc25B as a direct downstream substrate of PKA in mammals PMID:21212267
Cdc25b phosphatase is required for resumption of meiosis during oocyte maturation PMID:11912493
Cdc25A, or possibly other phosphatases, is able to functionally compensate for the loss of Cdc25B and Cdc25C in mice PMID:15767688
Shh contributes to CDC25B transcriptional activation in the neural tube both of chick and mouse embryos PMID:16564519
Cdc25b is regulated by Foxm1 during heart development. PMID:17366632
p38 regulates the timing of mitotic entry via modulation of Cdc25B activity under normal nonstress conditions PMID:17548358
Data show that the expression of cell cycle genes, Cdc25b, in the optic nerves is downregulated in a conditional ablation of Shh mutant mice. PMID:18625210
On the first mitotic division of mouse fertilized eggs, PRKACA regulates G2/M transition by phosphorylating CDC25B-Ser321 but not CDC25B-Ser229. PMID:18633139
Cdc25B subcellular localization determines its function during the process of maintaining germinal vesicle arrest in mouse oocytes PMID:19035343
Results suggest that Cdc25B is a direct target of PKA in prophase-arrested oocytes and that Cdc25B phosphorylation results in its inhibition and sequestration by the 14-3-3 protein. PMID:19223768