Recombinant Mouse Histone H2B type 1-M (Hist1h2bm)

Cloning the gene encoding the Mouse Hist1h2bm protein (2-126aa) into a plasmid vector leads to the formation of recombinant plasmid. The resulting recombination plasmid is transformed into e.coli cells. e.coli cells containing the recombinant plasmid survive in the presence of a specific antibiotic and are selected to be cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, the recombinant Mouse Hist1h2bm protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Mouse Hist1h2bm protein, demonstrating a purity exceeding 90%.