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Recombinant Mouse Glutamate dehydrogenase 1, mitochondrial (Glud1)

Recombinant Mouse Glutamate dehydrogenase 1, mitochondrial (Glud1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Glud1

Uniprot NO.

P26443

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

54-558

Target Protein Sequence

SEAAADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLK TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCVGV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKVYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPVVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.

Gene References into Functions

mRNA and protein levels of GluD1 were increased in fetal brain of foxg1(+/-) mice. mRNA and protein levels of GluD1 were decreased in adult brain of foxg1(+/-) mice. PMID:26443267
lack of glutamate oxidation in brain-specific GDH null CnsGlud1-/- mice resulted in a central energy-deprivation state with increased ADP/ATP ratios and phospho-AMPK in the hypothalamus. PMID:26440896
This study demonstrated a critical requirement for GluD1 in normal spine development in the cortex and hippocampus. PMID:25721396
The GDH activity in mice is highest in the liver with NAD(+) as a coenzyme and highest GDH activity was determined at a glutamate concentration of 10 mM. PMID:25124006
Age-related increases of glutamate were observed only in the striatum of the glutamate dehydrogenase 1 mice PMID:24442550
Glutamate hyperactivity caused gene expression changes in the hippocampus at all ages PMID:24593767
GluD1 regulates the connectivity of parallel fiber-interneuron synapses and promotes differentiation of interneurons. PMID:24872547
The GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. PMID:24295216
GluD1 is crucial for normal functioning of synapses and absence of GluD1 leads to specific abnormalities in learning and memory. PMID:23560106
Permissive levels of glutamate are required for the full development of glucose-stimulated insulin secretion and glutamate dehydrogenase plays an indispensable role in this process. PMID:22875990
Mice deficient in Glud1 exhibit deficient oxidative catabolism of glutamate in astrocytes, showing that GDH is required for Krebs cycle pathway; however, lack of GDH modifies metabolic handling of glutamate without altering synaptic transmission. PMID:22924626
These results suggest that GluD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex. PMID:22138648
Data show that Balb/c Glud1 transgenic mice had significantly increased depolarization-induced glutamate release compared with wild type littermates. PMID:21446915
In betaGlud1(-/-) mice, the reduced secretory capacity resulted in lower plasma insulin levels in response to both feeding and glucose load, while body weight gain was preserved PMID:19015267