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Recombinant Mouse G protein-coupled receptor kinase 5 (Grk5)

Recombinant Mouse G protein-coupled receptor kinase 5 (Grk5)

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MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Grk5

Purity

>85% (SDS-PAGE)

Target Names

Grk5

Uniprot NO.

Q8VEB1

Alternative Names

Grk5; Gprk5G protein-coupled receptor kinase 5; EC 2.7.11.16; G protein-coupled receptor kinase GRK5

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-590

Target Protein Sequence

MELENIVANT VLLKAREGGG GKRKGKSKKW KEILKFPHIS QCEDLRRTID RDYYSLCDKQ PIGRLLFRQF CETRPGLECY IQFLDLVAEY EITPDENLGA KGKEIMTKYL TPKSPVFIAQ VGQDLVSQTE KKLLQSPCKE LFSACAQSVH DYLKGDPFHE YLDSMYFDRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKRLEKK RIKKRKGESM ALNEKQILEK VNSQFVVNLA YAYETKDALC LVLTIMNGGD LKFHIYNMGN PGFEEERALF YAAEILCGLE DLHRENTVYR DLKPENILLD DYGHIRISDL GLAVKIPEGD LIRGRVGTVG YMAPEVLNNQ RYGLSPDYWG LGCLIYEMIE GQSPFRGRKE KVKREEVDRR VLETEEVYSS KFSEEAKSIC NMLLTKDSKQ RLGCQEEGAA EVKRHPFFRN MNFKRLEAGM LDPPFVPDPR AVYCKDVLDI EQFSTVKGVN LDHTDDDFYS KFSTGSVPIP WQNEMIETEC FKELNVFGPN GTLSPDLNRS QPPEPPKKGL FHRLFRRQHQ SNSKSSPTPK TSCNHRINSN HINSNSTGSS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).

Gene References into Functions

Along with other supporting evidence, the authors propose that GRK5 deficiency selectively renders basal forebrain cholinergic neurons more vulnerable to degeneration. PMID:27193825
Overall, these data suggest that GRK5 negatively regulates CXCL1/KC levels during bacterial pneumonia but that the role of GRK5 in the clinical outcome in this model is dependent on the bacterial dose. PMID:26975990
Generated mice with genetic knockout (KO) of the G protein-coupled receptor kinase (GRK) 5 gene by microinjection of TALEN mRNA. TALEN vectors were designed to target exons 1, 3, and 5 of the mouse GRK5 gene. PMID:27565865
These results of this study suggest that GRK5 deficiency indeed makes the mice more susceptible to wide range of behavioral impairments, including cognitive impairments. PMID:26778781
MOR phosphorylation is regulated by agonist-selective recruitment of distinct GRK isoforms that influence different opioid-related behaviors. Modulation of GRK5 function could serve as a new approach for preventing addiction to opioids. PMID:24629717
GRK5, acting in a kinase independent manner, is a facilitator of NFAT activity and part of a DNA-binding complex responsible for pathological hypertrophic gene transcription. PMID:25332207
influence of GRK5 on NF-kappaB expression and activity PMID:24174526
regulator of inflammation and thymic apoptosis in polymicrobial sepsis PMID:23485819
these data identified GRK5 as a positive regulator of insulin sensitivity. PMID:23111327
metoprolol is a biased ligand that selectively activates a G protein-independent and GRK5/beta-arrestin2-dependent pathway, and induces cardiac fibrosis. PMID:22888001
These results define myocyte GRK5 as a critical regulator of pathological cardiac growth after ventricular pressure overload, supporting its role as an endogenous (patho)-physiological HDAC kinase. PMID:22859683
these results suggest that GRK5 is an important regulator of adipogenesis and is crucial for the development of diet-induced obesity. PMID:22507984
GRK5 regulates IL-12p40 and G-CSF via a mechanism that is common to both MyD88 and TRIF. PMID:22078319
G protein-coupled receptor kinase-5 attenuates atherosclerosis by regulating receptor tyrosine kinases and 7-transmembrane receptors. PMID:22095977
GRK5 is a positive regulator of TLR4-induced IkappaBalpha-NFkappaB pathway as well as a key modulator of LPS-induced inflammatory response. PMID:20945396
GRK5 deficiency accelerates {beta}-amyloid accumulation in Tg2576 mice via impaired cholinergic activity PMID:21041302
GRK5 as a novel kinase of p53, as well as a negative regulator of p53-mediated signal transduction. PMID:20124405
Results identify GRK5/6 as novel kinases for the single transmembrane receptor LRP6 during Wnt signaling. PMID:19801552
Results demonstrate that both GRK2 and GRK5 are important and novel mediators of a non-traditional IkappaBalpha/NF-kappaB signalling pathway. PMID:19796012
GRK5 regulates pulmonary responses in a tissue- and receptor-specific manner but does not regulate peripheral cardiac muscarinic receptors. PMID:14565944
in both male and female mice, vascular smooth muscle-specific overexpression of GRK5 elevates blood pressure mediated by Gi and by beta1AR in males and Ang II receptors in females PMID:16103237
Taken together, these findings not only strongly support an important role of GRK5 deficiency in early Alzheimer's pathogenesis, but also promote the GRK5 knockout mouse as an additional model for early Alzheimer-related studies. PMID:17011668
These results demonstrate previously unknown crucial regulatory mechanisms that alter ARR/GRK expression levels in macrophages that might modify many, if not all, GPCR-mediated innate immune responses. PMID:18180038
GRK5 deficiency in vivo significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. PMID:18522748
Data show that GRK5 knockout female mouse displays increased hippocampal axonal defects and synaptic degenerative changes. PMID:18955119