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Recombinant Mouse Developmental pluripotency-associated protein 3 (Dppa3)

Recombinant Mouse Developmental pluripotency-associated protein 3 (Dppa3)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Dppa3

Purity

>85% (SDS-PAGE)

Target Names

Dppa3

Uniprot NO.

Q8QZY3

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-150

Target Protein Sequence

MEEPSEKVDP MKDPETPQKK DEEDALDDTD VLQPETLVKV MKKLTLNPGV KRSARRRSLR NRIAAVPVEN KSEKIRREVQ SAFPKRRVRT LLSVLKDPIA KMRRLVRIEQ RQKRLEGNEF ERDSEPFRCL CTFCHYQRWD PSENAKIGKN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

Gene References into Functions

The absence of the DPPA3 cleavage products adversely affects pre-implantation development and female fertility. PMID:29158485
Through an in-depth bioinformatic analysis of potential interactors, PGC7 was linked to critical cellular processes including translation, RNA processing, cell cycle, and regulation of heterochromatin structure. PMID:28712289
PRDM1 and STELLA, while overlapping in putative primordial germ cells, also co-localize in several other tissues PMID:27696611
The authors show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of maternal-to-zygotic transition. PMID:28323615
Data indicate STELLA's potential roles in building the fetal-placental connection. PMID:28322735
This study shows repression of the Dppa3 Locus in the absence of MAD2L2 embryonic stem cells. PMID:28330620
In-depth transcriptome analysis of this mESC state reveals Dppa3 as a direct downstream target of Tbx3.We show that Tbx3, a downstream target of Wnt signaling, fine tunes these divergent roles of Wnt signaling in mESCs PMID:26095607
Dppa3 has a critical role in generation of fully reprogrammed iPS cells and maintenance of Dlk1-Dio3 imprinting PMID:25613421
Stella protects maternal chromosomes from aberrant epigenetic modifications to ensure early embryogenesis. PMID:25694116
Chromocenter formation is impaired in Stella-null embryos. PMID:26325466
we propose that Dppa3 is involved in the Tet-mediated active demethylation process during reprogramming of primordial germ cells. PMID:23595900
PGC7 protects 5mC from Tet3-mediated conversion to 5hmC by binding to maternal chromatin containing dimethylated histone H3 lysine 9 (H3K9me2) in mice PMID:22722204
These Dppa3-MCM strains offer valuable opportunities to explore gene function in both loss-of-function and gain-of-function experiments at a variety of time points during germ cell development. PMID:21525417
DPPA3 is an essential factor for the production of functional oocytes through transcriptional repression and chromatin condensation. PMID:22034526
Data show that STELLA was found in both the nucleus and cytoplasm in a variety of cell types, both within and outside of the putative primordial germ cells (PGCs)trajectory. PMID:22019303
Data show that Stella is specifically expressed in preimplantation embryos and in the germline. PMID:21786403
embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage PMID:14654002
Dppa3 is expressed in germ cells and is a marker of cell pluripotency. PMID:14684992
a marker of pluripotency: Stella-positive ESCs were like the inner cell mass, Stella-negative cells were like the epiblast cells PMID:18940731

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Subcellular Location

Nucleus. Cytoplasm. Note=Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono-laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In 3.5 dpc blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.

Tissue Specificity

Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial

Database Links

UNIGENE: Mm.27982

KEGG: mmu:73708

STRING: 10090.ENSMUSP00000062832

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