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Recombinant Mouse DNA polymerase epsilon catalytic subunit A (Pole), partial

Recombinant Mouse DNA polymerase epsilon catalytic subunit A (Pole), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

Pole

Purity

>85% (SDS-PAGE)

Target Names

Pole

Uniprot NO.

Q9WVF7

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-2283

Target Protein Sequence

MVLRNSGRRH PEPGADGEGS RDDGPSSSVS ALKRLERSQW TDKMDLRFGF ERLKEPGERT GWLINMHPTE ILDEDKRLVS AVDYYFIQDD GSRFKVALPY MPYFYIAARK GCDREVSSFL SKKFQGKIAK LENVPKEDLD LPNHLVGLKR SYIKLSFHTV EDLVKVRKEI SPAVKKNREQ DHASDEYTTM LSSILQGGSV ITDEDETSKK IADQLDNIVD MREYDVPYHI RLSIDLRIHV AHWYNVRFRG NAFPVEITRR DDLVERPDPV VLAFDIETTK LPLKFPDAET DQIMMISYMI DGQGYLITNR EIVSEDIEDF EFTPKPEYEG PFCVFNEPDE VHLIQRWFEH IQETKPTIMV TYNGDFFDWP FVEARAAIHG LSMYQEIGFQ KDSQGEYKAP QCIHMDCLRW VKRDSYLPVG SHNLKAAAKA KLGYDPVELD PEDMCRMATE QPQTLATYSV SDAVATYYLY MKYVHPFIFA LCTIIPMEPD EVLRKGSGTL CEALLMVQAF HANIIFPNKQ EQEFNKLTDD GHMLDAETYV GGHVEALESG VFRSDIPCRF RMNPAAFDFL LQRVEKTMRH AIEEEEKVPV EQATNFQEVC EQIKTKLTSL KDVPNRIECP LIYHLDVGAM YPNIILTNRL QPSAIVDEAT CAACDFNKPG ASCQRKMAWQ WRGEFMPASR SEYHRIQHQL ESEKFPPLFP EGPARAFHEL SREEQAKYEK RRLADYCRKA YKKIHVTKVE ERLTTICQRE NSFYVDTVRA FRDRRYEFKG LHKVWKKKLS AAVEVGDASE VKRCKNMEIL YDSLQLAHKC ILNSFYGYVM RKGARWYSME MAGIVCFTGA NIITQARELI EQIGRPLELD TDGIWCVLPN SFPENFVIKT TNAKKPKLTI SYPGAMLNIM VKEGFTNHQY QELTEPSSLT YVTHSENSIF FEVDGPYLAM ILPASKEEGK KLKKRYAVFN EDGSLAELKG FEVKRRGELQ LIKIFQSSVF EAFLKGSTLE EVYGSVAKVA DYWLDVLYSK AANMPDSELF ELISENRSMS RKLEDYGEQK STSISTAKRL AEFLGDQMVK DAGLSCRYII SRKPEGSPVT ERAIPLAIFQ AEPTVRKHFL RKWLKSSSLQ DFDIRTILDW DYYIERLGSA IQKIITIPAA LQQVKNPVPR VKHPDWLHKK LLEKNDIYKQ KKISELFVLE GKRQIVMAQA SENSLSLCTP DMEDIGLTKP HHSTVPVATK RKRVWETQKE SQDIALTVPW QEVLGQPPSL GTTQEEWLVW LQFHKKKWQL QAQQRLALRK KQRLESAEDM PRLGPIREGP STGLGSFLRR TARSIMDLPW QIIQISETRQ AGLFRLWAII GNDLHCIKLS IPRVFYVNQR VAKAEDGPAY RKVNRALPRS NIVYNLYEYS VPEDMYQEHI NEINTELSVP DIEGVYETQV PLLFRALVQL GCVCVVNKQL TRHLSGWEAE TFALEHLEMR SLAQFSYLEP GSIRHIYLYH HTQGHKALFG VFIPSQRRAS VFVLDTVRSN QMPGLSALYS SEHSLLLDKV DPKLLPPPKH TFEVRAETNL KTICRAIQRF LLAYKEERRG PTLIAVQSSW ELCRLTSEIP VLEEFPLVPI RVADKISYAV LDWQRHGARR MIRHYLNLDL CLSQAFEMSR YFHIPVGNLP EDISTFGSDL FFARHLQHHN HLLWLSPTSR PDLGGKEADD NRLVMEFDDR ATVEINSSGC YSTVCVELDI QNLAVNTILQ SHHVNDMEGA GSMGISFDVI QQASLEDMVT GNQAASALAN YDETALCSST FRILKSMVVG WVKEITQYHN IYADNQVMHF YRWLQSPCSL LHDPALHRTL HNMMKKLFLQ LIAEFKRLGS SVVYANFNRI ILCTKKRRIE DALAYVEYIT NSIHSKEIFH SLTISFSRCW EFLLWMDPSN YGGIKGKVPS SIHCGQVKEQ DSQAREETDE EEEDKEKDEE EEGMGESEVE DLLENNWNIL QFLPQAASCQ SYFLMIVSAY IVAVYQSMKE ELRHSAPGST PVKRKGASQF SQESEGATGS LPGMITFSQD YVANELTQSF FTITQKIQKK VTGSRNTTEP SEMFPVLPGS HLLLNNPALE FIKYVCKVLS LDTNITNQVN KLNRDLLRLV DVGEFSEEAQ FRDPCHSYVL PEVICHSCNF CRDLDLCKDS SFSQDGAILP QWLCSNCQAP YDSSAIESAL VEALQRKLMA FTLQDLVCLK CRGMKETHMP VYCSCAGDFT LTIRTEVFME QIRIFQNIAK YYSMSYLQET IEWLLQTSPV SNC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

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■ Q&A

Regarding your protein CSB-EP894382MO, can you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, I want to have the tag removed from this protein. Have you ever performed tag-removal for this protein?
Thanks!

Thanks for your inquiry.
Expression Region: 1916-2283aa; Partial.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MDPSNYGGIKGKVPSSIHCGQVKEQDSQAREETDEEEEDKEKDEEEEGMGESEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKEELRHSAPGSTPVKRKGASQFSQESEGATGSLPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNTTEPSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDSSFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCSCAGDFTLTIRTEVFMEQIRIFQNIAKYYSMSYLQETIEWLLQTSPVSNC

Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% successfully.The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal fees and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.

Target Background

Function(From Uniprot)

Catalytic component of the DNA polymerase epsilon complex. Participates in chromosomal DNA replication. Required during synthesis of the leading DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.

Gene References into Functions

A missense mutation in Rev7 disrupts formation of Polzeta, impairing mouse development and repair of genotoxic agent-induced DNA lesions. PMID:24356953

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Subcellular Location

Nucleus.

Protein Families

DNA polymerase type-B family

Database Links

UNIGENE: Mm.35061

KEGG: mmu:18973

STRING: 10090.ENSMUSP00000007296

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