Recombinant Mouse DNA ligase 1 (Lig1), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Lig1

Uniprot NO.

P37913

Alternative Names

Lig1; Lig-1DNA ligase 1; EC 6.5.1.1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-916

Target Protein Sequence

MQRSIMSFFQ PTKEGKAKKP EKETPSSIRE KEPPPKVALK ERNQVVPESD SPVKRTGRKV AQVLSCEGED EDEAPGTPKV QKPVSDSEQS SPPSPDTCPE NSPVFNCSSP MDISPSGFPK RRTARKQLPK RTIQDTLEEQ NEDKTKTAKK RKKEEETPKE SLAEAEDIKQ KEEKEGDQLI VPSEPTKSPE SVTLTKTENI PVCKAGVKLK PQEEEQSKPP ARGAKTLSSF FTPRKPAVKT EVKQEESGTL RKEETKGTLD PANYNPSKNN YHPIEDACWK HGQKVPFLAV ARTFEKIEEV SARLKMVETL SNLLRSVVAL SPPDLLPVLY LSLNRLGPPQ QGLELGVGDG VLLKAVAQAT GRQLESIRAE VAEKGDVGLV AENSRSTQRL MLPPPPLTIS GVFTKFCDIA RLTGSASMAK KMDIIKGLFV ACRHSEARYI ARSLSGRLRL GLAEQSVLAA LAQAVSLTPP GQEFPTVVVD AGKGKTAEAR KMWLEEQGMI LKQTFCEVPD LDRIIPVLLE HGLERLPEHC KLSPGVPLKP MLAHPTRGVS EVLKRFEEVD FTCEYKYDGQ RAQIHVLEGG EVKIFSRNQE DNTGKYPDII SRIPKIKHPS VTSFILDTEA VAWDREKKQI QPFQVLTTRK RKEVDASEIQ VQVCLYAFDL IYLNGESLVR QPLSRRRQLL RENFVETEGE FVFTTSLDTK DTEQIAEFLE QSVKDSCEGL MVKTLDVDAT YEIAKRSHNW LKLKKDYLDG VGDTLDLVVI GAYLGRGKRA GRYGGFLLAA YDEESEELQA ICKLGTGFSD EELEEHHQSL QALVLPTPRP YVRIDGAVAP DHWLDPSIVW EVKCADLSLS PIYPAARGLV DKEKGISLRF PRFIRVRKDK QPEQATTSNQ VASLYRKQSQ IQNQQSSDLD SDVEDY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Gene References into Functions

Increased presynaptic glutamate release is a key early event resulting from Lgi1-deficiency, which likely contributes to epileptogenesis. PMID:26878798
Lig1 is not absolutely required for cellular DNA replication and repair in Lig1-null cell line. PMID:24726358
LigI has a highly specific role in CTG repeat maintenance in the maternal germline, involved in mediating CTG expansions and in the avoidance of maternal CTG contractions. PMID:21378394
DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability. PMID:11896201