Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Matrix metalloproteinase-13 ;MMP-13
Species
Mus musculus (Mouse)
Expression Region
105-472aa
Target Protein Sequence
YNVFPRTLKWSQTNLTYRIVNYTPDMSHSEVEKAFRKAFKVWSDVTPLNFTRIYDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFIVAAHELGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQFLYGPGDEDPNPKHPKTPEKCDPALSLDAITSLRGETMIFKDRFFWRLHPQQVEAELFLTKSFWPELPNHVDAAYEHPSRDLMFIFRGRKFWALNGYDILEGYPRKISDLGFPKEVKRLSAAVHFENTGKTLFFSENHVWSYDDVNQTMDKDYPRLIEEEFPGIGNKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPTNSILWC
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP177394m could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Mmp13.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP177394m could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Mmp13.
Description
The process of expressing this Mouse Mmp13 recombinant protein in E.coli expression system requires the following steps: use of competent E. coli cells to take up DNA sequence of interest, the exogenous plasmid is transferred into the cell, and the transcription and translation are directly replicated in the cell, selection of transformed E. coli using a selection marker, expansion of selected E. coli to a higher scale in appropriate culture media, such as classic LB options Growth Systems and lastly isolation and purification.And we finally get a Mmp13 protein with N-terminal 6xHis tag and it is determined by SDS-PAGE with a purity of 90%+.
MMP13 is a protein coding gene that encodes Collagenase 3 (Matrix metalloproteinase-13). According to some studies, MMP13 may have the following features.
MMP13 is a key target gene in the progression of osteoarthritis. MiR-9 was found to inhibit the secretion of type II collagen-targeted metalloproteinase MMP13 in isolated human chondrocytes. MMP13 may be a new tumor marker for the diagnosis of breast cancer. MMP13 is a stromal mediator that controls persistent angiogenesis in skin cancer. circRNA-CER participates in chondrocyte ECM degradation by regulating the expression of MMP13 as a competing endogenous RNA. MMP13 polymorphism reduces the risk of dental caries.
