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Recombinant Mouse Collagen alpha-1 (XVIII) chain (Col18a1)

Recombinant Mouse Collagen alpha-1 (XVIII) chain (Col18a1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Col18a1

Uniprot NO.

P39061

Alternative Names

Col18a1Collagen alpha-1(XVIII) chain [Cleaved into: Endostatin; Non-collagenous domain 1; NC1)]

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

27-1774

Target Protein Sequence

DGNS LSPLNPLVWL WPPKTSDSLE GPVSKPQNSS PVQSTENPTT HVVPQDGLTE QQTTPASSEL PPEEEEEEDQ KAGQGGSPAT PAVPIPLVAP AASPDMKEEN VAGVGAKILN VAQGIRSFVQ LWDEDSTIGH SAGTEVPDSS IPTVLPSPAE LSSAPQGSKT TLWLSSAIPS SPDAQTTEAG TLAVPTQLPP FQSNLQAPLG RPSAPPDFPG RAFLSSSTDQ GSSWGNQEPP RQPQHLEGKG FLPMTARSSQ QHRHSDVHSD IHGHVPLLPL VTGPLVTASL SVHGLLSVPS SDPSGQLSQV AALPGFPGTW VSHVAPSSGT GLSNDSALAG NGSLTSTSRC LPLPPTLTLC SRLGIGHFWL PNHLHHTDSV EVEATVQAWG RFLHTNCHPF LAWFFCLLLA PSCGPGPPPP LPPCRQFCEA LEDECWNYLA GDRLPVVCAS LPSQEDGYCV FIGPAAENVA EEVGLLQLLG DPLPEKISQI DDPHVGPAYI FGPDSNSGQV AQYHFPKLFF RDFSLLFHVR PATEAAGVLF AITDAAQVVV SLGVKLSEVR DGQQNISLLY TEPGASQTQT GASFRLPAFV GQWTHFALSV DGGSVALYVD CEEFQRVPFA RASQGLELER GAGLFVGQAG TADPDKFQGM ISELKVRKTP RVSPVHCLDE EDDDEDRASG DFGSGFEESS KSHKEDTSLL PGLPQPPPVT SPPLAGGSTT EDPRTEETEE DAAVDSIGAE TLPGTGSSGA WDEAIQNPGR GLIKGGMKGQ KGEPGAQGPP GPAGPQGPAG PVVQSPNSQP VPGAQGPPGP QGPPGKDGTP GRDGEPGDPG EDGRPGDTGP QGFPGTPGDV GPKGEKGDPG IGPRGPPGPP GPPGPSFRQD KLTFIDMEGS GFSGDIESLR GPRGFPGPPG PPGVPGLPGE PGRFGINGSY APGPAGLPGV PGKEGPPGFP GPPGPPGPPG KEGPPGVAGQ KGSVGDVGIP GPKGSKGDLG PIGMPGKSGL AGSPGPVGPP GPPGPPGPPG PGFAAGFDDM EGSGIPLWTT ARSSDGLQGP PGSPGLKGDP GVAGLPGAKG EVGADGAQGI PGPPGREGAA GSPGPKGEKG MPGEKGNPGK DGVGRPGLPG PPGPPGPVIY VSSEDKAIVS TPGPEGKPGY AGFPGPAGPK GDLGSKGEQG LPGPKGEKGE PGTIFSPDGR ALGHPQKGAK GEPGFRGPPG PYGRPGHKGE IGFPGRPGRP GTNGLKGEKG EPGDASLGFS MRGLPGPPGP PGPPGPPGMP IYDSNAFVES GRPGLPGQQG VQGPSGPKGD KGEVGPPGPP GQFPIDLFHL EAEMKGDKGD RGDAGQKGER GEPGAPGGGF FSSSVPGPPG PPGYPGIPGP KGESIRGPPG PPGPQGPPGI GYEGRQGPPG PPGPPGPPSF PGPHRQTVSV PGPPGPPGPP GPPGAMGASA GQVRIWATYQ TMLDKIREVP EGWLIFVAER EELYVRVRNG FRKVLLEART ALPRGTGNEV AALQPPLVQL HEGSPYTRRE YSYSTARPWR ADDILANPPR LPDRQPYPGV PHHHSSYVHL PPARPTLSLA HTHQDFQPVL HLVALNTPLS GGMRGIRGAD FQCFQQARAV GLSGTFRAFL SSRLQDLYSI VRRADRGSVP IVNLKDEVLS PSWDSLFSGS QGQLQPGARI FSFDGRDVLR HPAWPQKSVW HGSDPSGRRL MESYCETWRT ETTGATGQAS SLLSGRLLEQ KAASCHNSYI VLCIENSFMT SFSK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.; May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.; Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Inhibits VEGFA isoform VEGF165-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5. May negatively regulate the activity of homotrimeric non-collagenous domain 1.

Gene References into Functions

This study examined the staining levels of proteasomal and autophagy markers in the RPE of different ages of the Col18a1 (-/-) mice. Eyes from 3, 6-7, 10-13 and 18 months old mice were enucleated and embedded in paraffin according to the routine protocol. PMID:27125427
Kidney fibrosis in aging may represent a natural outcome of upregulated endostatin (EST) and transglutaminase 2 (TG2), but more likely it appears to be a result of cumulative stresses occurring on the background of synergistically acting geronic (aging) proteins, EST and TG2. PMID:27165830
Specific collagen XVIII isoforms promote adipose tissue accrual via mechanisms determining PMID:25024173
There are correlations between the elevation of endostatin levels and parallel microvascular rarefaction and induction of renal fibrosis in aging mice. PMID:24727495
An excess of the collagen XVIII Tsp-1 domain is deleterious in the eye, possibly by impairing certain functions of the full-length molecule. PMID:24135756
The results of the present study indicate endostatin promotes soft callus formation but inhibits callus remodeling during fracture healing most probably by an inhibition of angiogenesis. PMID:23720153
Type XVIII collagen is an important functional component of the liver matrix microenvironment and is crucial for hepatocyte survival during injury and stress. PMID:23580202
Data indicate that delivery by attenuated Salmonella of the combination therapeutic plasmid simultaneously knocked down the expression of Stat3 and resulted in over-expression of Endostatin, which synergistically inhibited prostate cancer growth. PMID:23463096
Endostatin lowers blood pressure via nitric oxide and prevents hypertension associated with VEGF inhibition PMID:22733742
Women with early-onset, but not late-onset preeclampsia have higher levels of endostatin than healthy pregnant women in corresponding lengths of gestation. PMID:19564642
Lack of collagen XVIII long isoforms affects kidney podocytes, whereas the short form is needed in the proximal tubular basement membrane PMID:21193414
Data show that Col18-deficiency causes hypertriglyceridemia in mice. PMID:21085708
In vivo, Collagen XVIII/endostatin does not play an indispensable role in skeletal development PMID:19763371
the local synthesis of a 28-kDa ES-related fragment following acute kidney injury and suggest its role in endothelium survival PMID:20110665
Lack of collagen XVIII/endostatin results in eye abnormalities. PMID:11927538
Endostatin gene transfer inhibits joint angiogenesis and pannus formation in inflammatory arthritis PMID:11991745
Endostatin expression effectively inhibits mammary neoplasms growth in nude mice PMID:12673115
Col18a1 knockout mice have iris and ciliary body defects. PMID:14525950
Col18a1 is essential for vision and retinal pigment epithelial function. PMID:14685281
there is a putative integrin-binding sequence with anti-migratory activity within endostatin PMID:14973128
Collagen XVIII and enamelysin were co-localized in the developing enamel matrix and stratum intermedium and in the enamel-like tumor matrix of odontogenic tumors. PMID:15296943
Studies in knockout mice identify a previously unrecognized function for collagen XVIII in vascular permeability. Loss of collagen XVIII enhances angiogenesis & vascular permeability in atherosclerosis by distinct gene-dose-dependent mechanisms. PMID:15313955
collagen XVIII's C-terminal endostatin domain harbors a prominent tissue-binding site, which can bind in the absence of heparan sulfates in situ PMID:15614762
In knockout mice, a widening of the epidermal basement membrane (BM) zone of the skin was observed in electron microscopy. PMID:15632014
angiostatic function is heparan sulfate-dependent, and in situ-binding of endostatin to endothelial cells is increased by heparan sulfates PMID:15694132
The p.D104N allele was found in trans with a pathogenic frameshift mutation in a healthy carrier of Knobloch syndrome, showing it is not very harmful. It may be a modifier or at-risk allele. PMID:15714516
Collagen XVIII is localized in various basement membranes and is highly expressed throughout the connective tissue core of the endocardial cushions and embryonic atrioventricular valve leaflets; it may have a role in cardiac valve morphogenesis. PMID:16170784
Exposure to hypoxia resulted in organ-specific, long-term increase of 24 & 26 kDa endostatin fragments in mouse aorta & lung. PMID:16465514
Proteolytic matrix fragment endostatin(ES) is prominent vasorelaxing agent. Because ES is constantly released into the blood, it is novel regulator of blood pressure and represents an interesting pharmacological target. PMID:16574906
Data show that upon experimental and human renal ischemia/reperfusion, microvascular basement membrane (BM) heparan sulfate proteoglycans (collagen 18alpha1, agrin and perlecan) are modified to bind L-selectin and monocyte chemoattractant protein-1. PMID:17525255
following resection of cirrhotic liver, regeneration capacity is depressed and high endostatin levels are independent of hepatic regeneration PMID:17574579
Results show that endogenous endostatin and its related peptides are widely distributed in all in vivo tumor types tested, but not in most of the cultured tumor cell lines. PMID:17727259
Local synthesis of a 30 kDa endostatin-related fragment following acute renal failure. Role in the modulation of renal capillary density. PMID:17803469
Endostatin binding to ovarian cancer cells inhibits peritoneal attachment and dissemination PMID:18006826
D104N polymorphism of the COL18A gene may be an important inherited determinant of the LC susceptibility. PMID:18054814
Inhibition of tumor angiogenesis by endostatin at an early stage in skin tumor development and an inhibition of lymph node metastasis. PMID:18089781
study shows that a lack of collagen XVIII accelerates wound vascularisation and the rate of cutaneous wound healing whereas an excess of endostatin leads to delayed wound healing PMID:18455382
These data indicate that collagen XVIII may play an important role in corneal reinnervation after wounding. PMID:18840438
Report effects of endostatin production on oncogenicity and metastatic activity of HPV16-transformed mouse cells: role of interleukin 1alpha. PMID:19513570

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Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane.; [Non-collagenous domain 1]: Secreted, extracellular space, extracellular matrix, basement membrane. Secreted.; [Endostatin]: Secreted. Secreted, extracellular space, extracellular matrix, basement membrane.

Protein Families

Multiplexin collagen family

Tissue Specificity

Expressed in liver, kidney, lung, skeletal muscle and testis.

Database Links

UNIGENE: Mm.4352

KEGG: mmu:12822

STRING: 10090.ENSMUSP00000101049

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