Recombinant Mouse Cerebellin-1 (Cbln1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Cbln1

Purity

>85% (SDS-PAGE)

Target Names

Cbln1

Uniprot NO.

Q9R171

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

22-193

Target Protein Sequence

QNETEPIVL EGKCLVVCDS NPTSDPTGTA LGISVRSGSA KVAFSAIRST NHEPSEMSNR TMIIYFDQVL VNIGNNFDSE RSTFIAPRKG IYSFNFHVVK VYNRQTIQVS LMLNGWPVIS AFAGDQDVTR EAASNGVLIQ MEKGDRAYLK LERGNLMGGW KYSTFSGFLV FPL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the matching and maintenance of pre- and post-synaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses. Plays a role as a synaptic organizer that acts bidirectionally on both pre- and post-synaptic components. On the one hand induces accumulation of synaptic vesicles in the pre-synaptic part by binding with NRXN1 and in other hand induces clustering of GRID2 and its associated proteins at the post-synaptic site through association of GRID2. NRXN1-CBLN1-GRID2 complex directly induces parallel fiber protrusions that encapsulate spines of Purkinje cells leading to accumulation of GRID2 and synaptic vesicles. Required for CBLN3 export from the endoplasmic reticulum and secretion. NRXN1-CBLN1-GRID2 complex mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis. Essential for long-term maintenance but not establishment of excitatory synapses. Inhibits the formation and function of inhibitory GABAergic synapses in cerebellar Purkinje cells.; The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.

Gene References into Functions

Results show that Cbln1, Cbln2, and Cbln4 are abundantly expressed in nearly all brain regions, but exhibit strikingly different expression patterns and developmental dynamics. Using newly generated knockin reporter mice for Cbln2 and Cbln4, we find that Cbln2 and Cbln4 are not universally expressed in all neurons, but only in specific subsets of neurons. PMID:28714144
results suggest that gene and seizure interactions in ventral tegmental area glutamatergic neurons impair sociability by downregulating Cbln1, a key node in the expanding protein interaction network of autism genes PMID:28297715
Despites its well known role in motor coordination and motor learning, whether and how the cerebellum is involved in cognitive functions remains less clear. Cerebellin 1 (Cbln1) is highly expressed in the cerebellum and serves as an essential synaptic organizer. Although genes encoding Cbln1 and its receptor are associated with many psychiatric disorders, it remains unknown whether such cognitive impairments are caused by PMID:27852787
amplitude and frequency of mIPSCs were increased in cbln1-null cerebellar slices. PMID:24467251
This study provides evidence for involvement of Cbln1 in nociception and provides a platform for continued exploration of involvement of novel neuropeptides in the regulation of nociceptive transmission PMID:24749662
that neurons expressing Cblns 1, 2, and 4 tend to occupy different laminar positions within the dorsal spinal cord PMID:23853053
extracerebellar structures or remaining PF-PC synapses in cbln1(-/-) mice may be sufficient for the expression, maintenance, and extinction of its memory trace. PMID:24298240
Cbln1 released from parallel fiber Purkinje cells induces dynamic structural changes in parallel fibers. PMID:23141067
[review] The Cbln1-GluRdelta2 receptor complex is located at the cleft of parallel fiber-Purkinje cell synapses and bidirectionally regulates both presynaptic and postsynaptic differentiation. PMID:20535596
GluRdelta1 stimulated inhibitory synapse formation in the presence of Cbln1 or Cbln2 PMID:22191730
Data show that Cbln1 and Cbln2, but not Cbln4, specifically bind to neurexin 1alpha and -beta and induce synaptogenesis in cerebellar, hippocampal and cortical neurons in vitro. PMID:21410790
These results suggest that Cbln1 and Cbln2 play a role in synapse formation of cortical neurons by interacting with NRXNs containing S4. PMID:21356198
Otolin 1 interacts with otoconial matrix protein Otoconin-90 and Cerebellin-1 in the inner ear. PMID:20856818
Our observation that loss of Cbln1 leads to increased numbers of striatal medium spiny neurons' dendritic spines suggests that modulating thalamostriatal Cbln1 may be a novel means of improving symptoms or slowing progression in Parkinson's disease. PMID:20503425
the flap loop (Arg321-Trp339) in the NTD of GluD2 (GluD2-NTD) was identified as a crucial region for the binding to Cbln1 and the induction of presynaptic differentiation. PMID:20599760
Results suggest that GluRdelta2 mediates cerebellar synapse formation by interacting with presynaptic NRXNs through Cbln1. PMID:20537373
study found that Cbln1 binds directly to the N-terminal domain of glutamate receptor delta2 (GluD2); results indicate that the Cbln1-GluD2 complex is a unique synapse organizer that acts bidirectionally on both pre- and postsynaptic components PMID:20395510
the cleavages required to produce cerebellin influence the subunit stoichiometry of Cbln1 complexes PMID:16135095
In the developing brain, Cbln1 mRNA appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. PMID:16930405
Cbln1 is required for endoplasmic reticulum export and secretion of Cbln3. PMID:17030622
During development of cbln1-lacZ mice, beta-galactosidase staining was first observed in proliferating granule cell precursors prior to synaptogenesis and thereafter in maturing and adult granule cells. PMID:18001291
Cbln1 is a unique synapse organizer that is required not only for the normal development of parallel fiber-Purkinje cell synapses but also for their maintenance in the mature cerebellum both in vitro and in vivo. PMID:18524896
These results indicate that Cbln1, released from granule cells as hexamers, specifically binds to a putative receptor located at the postsynaptic sites of parallel-Purkinje cell synapses. PMID:19200061
immunohistochemical localization suggests the basis for anatomical convergence of the Cbln-GluRdelta2 signaling pathway in parallel fiber-Purkinje cell synapses PMID:19250438
Data show that Cbln1 undergoes anterograde transport from granule cells to Purkinje cells and Bergmann glia and enters the endolysosomal trafficking system, suggesting that Cbln1 exerts its activity on or within Purkinje cells and Bergmann glia. PMID:19344768
Activity-dependent downregulation of Cbln1 may serve as a new presynaptic mechanism by which parallel fibers-Purkinje cell synapses adapt to chronically elevated activity, thereby maintaining homeostasis. PMID:19403810

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Subcellular Location

Secreted. Cell junction, synapse, postsynaptic cell membrane.

Tissue Specificity

Expressed and secreted by presynaptic neurons, such as cerebellar granule cells. Following secretion, the protein binds to GRID2 on the postsynaptic Purkinje cell membranes. Expressed at the highest level in the cerebellar cortex. High levels are also see

Database Links

UNIGENE: Mm.4880

KEGG: mmu:12404

STRING: 10090.ENSMUSP00000034076

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