Home
Product
Protein
Recombinant Proteins
Recombinant Mouse AT-rich interactive domain-containing protein 1A (Arid1a), partial

Recombinant Mouse AT-rich interactive domain-containing protein 1A (Arid1a), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Arid1a

Uniprot NO.

A2BH40

Alternative Names

Arid1a; Baf250; Baf250a; Osa1; Smarcf1; AT-rich interactive domain-containing protein 1A; ARID domain-containing protein 1A; BRG1-associated factor 250; BAF250; BRG1-associated factor 250a; BAF250A; Osa homolog 1; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related; matrix-associated; actin-dependent regulator of chromatin subfamily F member 1

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

2-2283

Target Protein Sequence

AAQVAPAAA SSLGNPPPPP SELKKAEQQQ REEAGGEAAA AAAERGEMKA AAGQESEGPA VGPPQPLGKE LQDGAESNGG GGGGGAGSGG GPGAEPDLKN SNGNAGPRPA LNNNLPEPPG GGGGGGSSSS DGVGAPPHSA AAALPPPAYG FGQAYGRSPS AVAAAAAAVF HQQHGGQQSP GLAALQSGGG GGLEPYAGPQ QNSHDHGFPN HQYNSYYPNR SAYPPPPQAY ALSSPRGGTP GSGAAAAAGS KPPPSSSASA SSSSSSFAQQ RFGAMGGGGP SAAGGGTPQP TATPTLNQLL TSPSSARGYQ GYPGGDYGGG PQDGGAGKGP ADMASQCWGA AAAAAAAAAA VSGGAQQRSH HAPMSPGSSG GGGQPLARTP QSSSPMDQMG KMRPQPYGGT NPYSQQQGPP SGPQQGHGYP GQPYGSQTPQ RYPMTMQGRA QSAMGSLSYA QQIPPYGQQG PSAYGQQGQT PYYNQQSPHP QQQPPYAQQP PSQTPHAQPS YQQQPQTQQP QLQSSQPPYS QQPSQPPHQQ SPTPYPSQQS TTQQHPQSQP PYSQPQAQSP YQQQQPQQPA SSSLSQQAAY PQPQPQQSQQ TAYSQQRFPP PQELSQDSFG SQASSAPSMT SSKGGQEDMN LSLQSRPSSL PDLSGSIDDL PMGTEGALSP GVSTSGISSS QGEQSNPAQS PFSPHTSPHL PGIRGPSPSP VGSPASVAQS RSGPLSPAAV PGNQMPPRPP SGQSDSIMHP SMNQSSIAQD RGYMQRNPQM PQYTSPQPGS ALSPRQPSGG QMHSGVGSYQ QNSMGSYGPQ GSQYGPQGGY PRQPNYNALP NANYPNAGMA GSMNPMGAGG QMHGQPGIPP YGTLPPGRMA HASMGNRPYG PNMANMPPQV GSGMCPPPGG MNRKTQESAV AMHVAANSIQ NRPPGYPNMN QGGMMGTGPP YGQGINSMAG MINPQGPPYP MGGTMANNSA GMAASPEMMG LGDVKLTPAT KMNNKADGTP KTESKSKKSS SSTTTNEKIT KLYELGGEPE RKMWVDRYLA FTEEKAMGMT NLPAVGRKPL DLYRLYVSVK EIGGLTQVNK NKKWRELATN LNVGTSSSAA SSLKKQYIQC LYAFECKIER GEDPPPDIFA AADSKKSQPK IQPPSPAGSG SMQGPQTPQS TSSSMAEGGD LKPPTPASTP HSQIPPLPGM SRSNSVGIQD AFPDGSDPTF QKRNSMTPNP GYQPSMNTSD MMGRMSYEPN KDPYGSMRKA PGSDPFMSSG QGPNGGMGDP YSRAAGPGLG SVAMGPRQHY PYGGPYDRVR TEPGIGPEGN MGTGAPQPNL MPSTPDSGMY SPSRYPPQQQ QQQQQQHDSY GNQFSTQGTP SSSPFPSQQT TMYQQQQQNY KRPMDGTYGP PAKRHEGEMY SVPYSAGQGQ PQQQQLPAAQ SQPASQPQAA QPSPQQDVYN QYSNAYPASA TAATDRRPAG GPQNQFPFQF GRDRVSAPPG SSAQQNMPPQ MMGGPIQASA EVAQQGTMWQ GRNDMTYNYA NRQNTGSATQ GPAYHGVNRT DEMLHTDQRA NHEGPWPSHG TRQPPYGPSA PVPPMTRPPP SNYQPPPSMP NHIPQVSSPA PLPRPMENRT SPSKSPFLHS GMKMQKAGPP VPASHIAPTP VQPPMIRRDI TFPPGSVEAT QPVLKQRRRL TMKDIGTPEA WRVMMSLKSG LLAESTWALD TINILLYDDN SIMTFNLSQL PGLLELLVEY FRRCLIEIFG ILKEYEVGDP GQRTLLDPGR FTKVYSPAHT EEEEEEHLDP KLEEEEEEGV GNDEEMAFLG KDKPSSENNE EKLVSKFDKL PVKIVQRNDP FVVDCSDKLG RVQEFDSGLL HWRIGGGDTT EHIQTHFESK IELLPSRPYV PCPTPPRKHL TTVEGTPGTT EQEGPPPDGL PEKRITATMD DMLSTRSSTL TDEGAKSAEA TKESSKFPFG ISPAQSHRNI KILEDEPHSK DETPLCTLLD WQDSLAKRCV CVSNTIRSLS FVPGNDFEMS KHPGLLLILG KLILLHHKHP ERKQAPLTYE KEEEQDQGVS CDKVEWWWDC LEMLRENTLV TLANISGQLD LSPYPESICL PVLDGLLHWA VCPSAEAQDP FSTLGPNAVL SPQRLVLETL SKLSIQDNNV DLILATPPFS RLEKLYSTMV RFLSDRKNPV CREMAVVLLA NLAQGDSLAA RAIAVQKGSI GNLLGFLEDS LAATQFQQSQ ASLLHMQNPP FEPTSVDMMR RAARALLALA KVDENHSEFT LYESRLLDIS VSPLMNSLVS QVICDVLFLI GQS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Gene References into Functions

ARID1A regulates expression of SOX9, activation of the mTOR pathway, and differentiation of pancreatic ductal cells. ARID1A inhibits formation of pancreatic ductal adenocarcinomas from intraductal papillary mucinous neoplasms in mice with pancreatic expression of activated KRAS and is down-regulated in IPMN and PDAC tissues from patients. PMID:29604291
Our results identified PIK3IP1 as a novel target of ARID1A and PGR in the murine uterus. PMID:29289536
Mice with liver-specific homozygous or heterozygous Arid1a loss were resistant to tumor initiation while ARID1A overexpression accelerated initiation. PMID:29136504
Loss of HDAC-mediated repression and gain of NF-kappaB activation underlie cytokine induction in ARID1A- and PIK3CA-mutation-driven ovarian cancer. PMID:27681437
ARID1A mutation inactivates the apoptosis-promoting function of p53 by upregulating HDAC6, indicating that pharmacological inhibition of HDAC6 is a therapeutic strategy for ARID1A-mutated cancers. PMID:28737768
ARID1A normally targets SWI/SNF complexes to enhancers. PMID:27941798
Consistent with the latter, Arid1a reexpression in tumor cells led to increased p21 (Cdkn1a) expression and dramatic accumulation of cells in G2 phase of the cell cycle. These results also indicate a potential opportunity for therapeutic intervention in ARID1A-deficient human breast cancer subtypes that retain one intact copy of the gene and also maintain wild-type TRP53 activity PMID:27280691
regulates embryo implantation and development of a functional placenta PMID:26962117
The Arid1a loss reprograms chromatin to restrict promoter access by transcription factors such as C/ebpalpha, which enforces differentiation, and E2F4, which suppresses cell-cycle re-entry. PMID:27044474
This study provides an alternative mechanism by which Arid1a deficiency contributes to hepatocellular carcinoma tumorigenesis. PMID:26569409
ARID1A positively regulates Klf15 expression with PGR to inhibit epithelial proliferation at peri-implantation. Our results suggest that Arid1a has a critical role in modulating epithelial proliferation which is a critical requisite for fertility PMID:26378916
Our results indicate that the Arid1a tumour suppressor gene has a key role in regulating ovarian endometrioid carcinoma differentiation PMID:26279473
ARID1A and PIK3CA mutations cooperate to promote tumour growth through sustained IL-6 overproduction. PMID:25625625
BAF250a mediates esBAF and polycomb recessive complex functions to establish the poised chromatin configuration in embryonic stem (ES) cells, which is essential for ES cell differentiation. PMID:26070559
The Baf250a orchestrated a genetic program that repressed the expression of Nkx2.5 in the SAN through Tbx3 and HDAC3 PMID:25145359
Results demonstrate that inactivation of Arid1a alone is insufficient for tumor initiation but it requires additional genetic alteration(s) such as Pten deletion to drive tumorigenesis. PMID:24899687
Loss of ARID1a-DNA binding led to a convergence of heart and vascular defects in mutant embryos, and the sum of defects culminated in ischemic arrest around midgestation. PMID:23129809
Results thus identify BAF250a-mediated chromatin remodeling as an essential epigenetic mechanism mediating CPC differentiation. PMID:22621927
The patterns of expression of ARID1A and ARID1B genes through various mouse embryonic stages, was examined. PMID:21647563
Data show that the Baf250a(E2E3/E2E3) FL-derived stroma, in contrast, exhibits a hemopoiesis-supporting potential superior to the developmentally matched controls. PMID:20522713
Results provide a direct biological basis to support the implication from tumor tissue screens that deficiency of p270 plays a causative role in carcinogenesis. PMID:16230384
BAF250a is a key component of the gene regulatory machinery in embryonic stem cells controlling self-renewal, differentiation, and cell lineage decisions. PMID:18448678