Recombinant Mouse 14-3-3 protein epsilon (Ywhae)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ywhae

Uniprot NO.

P62259

Alternative Names

Ywhae14-3-3 protein epsilon; 14-3-3E

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-255

Target Protein Sequence

MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.

Gene References into Functions

Rod photoreceptors are enriched in YWHAE (14-3-3 epsilon). Immunohistochemistry revealed that 14-3-3 epsilon and 14-3-3 zeta exhibit unique distributions in photoreceptors with 14-3-3 epsilon restricted to the inner segment and 14-3-3 zeta localized to the outer segment. PMID:29486162
Authors used 14-3-3epsilon (Ywhae) flox mice and found that 14-3-3epsilon deficiency results in an increase in neurite formation. PMID:28173130
The complete ablation of the 14-3-3epsilon protein results in multiple defects in neuropsychiatric behaviors in mice. PMID:27845227
14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID:26208633
Data indicate that 14-3-3epsilon is required for the mitotic entry in the fertilized mouse eggs and responsible for sequestering the CDC25B in cytoplasm. Its binding to CDC25B-S321 phosphorylated by PKA induces mitotic arrest. PMID:25059436
Ser321 of Cdc25B is the specific binding site for 14-3-3epsilon binding. PMID:23326474
14-3-3epsilon deletion did not appear to induce compensation by other 14-3-3 isoforms but led to ventricular noncompaction, resulting from a selective reduction in compact myocardium thickness. PMID:23071090
14-3-3epsilon haploinsufficiency decreased the incorporation of expanded ATXN1 into its large toxic complexes in the cerebellum but not in the brainstem, and the distribution of ATXN1's small and large native complexes differed significantly between two regions PMID:21245341
14-3-3 epsilon belongs to a regulatory protein family involved in important cellular processes, including those leading to neurodegenerative diseases, and thus its increased expression suggests a role of this protein in tuning microglia activation PMID:22065591
The finding of this study suggested that dysfunction of the TH neuronal network caused by the deficit of 14-3-3 epsilon may have been involved in the pathophysiology of schizophrenia and correlated with a dysfunction in the DISC1 complex. PMID:21458426
14-3-3epsilon interacts directly with PDX-1 to regulate its cellular distribution in pancreatic beta cells. PMID:21287435
overexpression of 14-3-3epsilon led to resistance to both rotenone and 1-methyl-4-phenylpyridinium (MPP(+)), while other isoforms were not protective against both toxins. PMID:21152247
The appearance of 14-3-3 proteins in the cerebrospinal fluid is characteristic of some neurodegenerative conditions PMID:11784696
14-3-3epsilon is important for neuronal migration by binding to NUDEL and provide a molecular explanation for the differences in severity of human neuronal migration defects with 17p13.3 deletions PMID:12796778
14-3-3 adapter proteins may have a role in regulating CD81 signaling, possibly dependent on cellular redox PMID:14966136
HSF1 associates with ERK1 and 14-3-3epsilon during heat shock to modulate the amplitude of the response and lead to efficient termination of HSP expression on resumption of growth conditions PMID:15364926
UBPY is catalytically inhibited in a phosphorylation-dependent manner by 14-3-3s during the interphase, and this regulation is cancelled in the M phase. PMID:17720156
aldosterone-induced 14-3-3 isoforms, beta and epsilon, interact with phospho-Nedd4-2 as an obligatory heterodimer, blocking its interaction with ENaC and thereby increasing apical ENaC density and sodium transport. PMID:18687683