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Recombinant Miscanthus streak virus Replication-associated protein (C1/C2)

Recombinant Miscanthus streak virus Replication-associated protein (C1/C2)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

C1/C2

Purity

>85% (SDS-PAGE)

Target Names

C1/C2

Uniprot NO.

Q67590

Species

Miscanthus streak virus (isolate 91) (MiSV)

Source

Yeast

Expression Region

1-348

Target Protein Sequence

MRAPASSAAS NRPGPSNHPT PRWNSKQFFL TYPHCNLTPS ELMKELFSRL TEKIPGYIKV SQEFHKDGDP HLHVLIQLNT KLCTRNPKFF DVQGFHPNIQ PVRDAEKVFG YISKTNGDSD EMGELQLRIK KPEKPTRDQR MAMIIASSTN RNEYLSMVRK EFPFDWAIRL QQFEYSAAAL FTEPPPVYQS PFPNEQIVCP PELVDIIDQE WNQQPNGPRR PRSIYICGPS RTGKTTWARN IGRHNYYNST VDFTHYDKDA IYNVIDDVPF KFLPQWKALV GAQRDYIVNP KYGKKKKIPG GIPSIILTND DEDWIKDMKP AQVEYLHANA HVHYMYEGQK FYVLPAEE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. Acts as an inhibitor of C-sense gene transcription.

Subcellular Location

Host nucleus.

Protein Families

Geminiviridae Rep protein family

Database Links

KEGG: vg:932215

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