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Recombinant Meyerozyma guilliermondii Ubiquitin-like protein ATG12 (ATG12)

Recombinant Meyerozyma guilliermondii Ubiquitin-like protein ATG12 (ATG12)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ATG12

Uniprot NO.

A5DK05

Alternative Names

ATG12; PGUG_03606Ubiquitin-like protein ATG12; Autophagy-related protein 12

Species

Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)

Source

Yeast

Expression Region

1-213

Target Protein Sequence

MTPPTSNSSM VDSLSSWVAE SLSSDMRNNY STSVGKGRAK LACGFYQERW CIDALQKVVS INEILCGMSF IHSEPDSDSS TTSADSNTLS QHVDKTSVPL DTSIVVKRLP EVEQRQWHKT IKPLPATKIT IRLVPIGSTR AITPKVLQVS SDQTVAVLMR FIAKKLRIST DSVYMYIHNT FQPTPDERLG DLYDQFRTNQ ELIFNYCNTV AFG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate functions as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes.

Subcellular Location

Preautophagosomal structure membrane; Peripheral membrane protein.

Protein Families

ATG12 family

Database Links

KEGG: pgu:PGUG_03606

STRING: 4929.A5DK05

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