Recombinant Methanosarcina barkeri DNA ligase 1 (lig1), partial

Product Details

Abbreviation
lig1
Purity
>85% (SDS-PAGE)
Target Names
lig1
Uniprot NO.
Alternative Names
lig1; Mbar_A1643DNA ligase 1; EC 6.5.1.1; Polydeoxyribonucleotide synthase [ATP] 1
Species
Methanosarcina barkeri (strain Fusaro / DSM 804)
Source
Yeast
Expression Region
1-549
Target Protein Sequence
MERKEIKDSA YLFLGSIGPA FENITLGIGD KLALKAIAGA YRVSEEEVKK RYSRTGDLGD VAFELNQGEE KSLAIDEVFG SLKEIKEASG KGSQEEKTGL LSSILQRASP KEGKYIVRIV LGKLRLGFGD QFLLEAFSIA FTGDKKYVVK IKESYSVCTD IGELAESLAE QGPKALGHFS IKLGRPVRSM LAQRVKTFEE LEERIPGKKA AEEKYDGERV QIHKNGEEIK AFSRRLEDIT AQYPDVIEAV RKGILAKKIV LDGEIIAYVE GGKANDSTGE FYSFQRLMKR RRKYEVQKYT EICPVAVFFF DILYLEGNSL LKKPYPERRA ILEEHVKESE ILHLARRIVT DNLEEIEDFF NEALEKRLEG IIIKSMGRNS AYEAGKRSWF WLKWKEEYAS GMRETFDLAI IGKYYGRGKR KGSFGALLCA ILNGEEQRFE TFTKVGTGFT EADAKEIDSL LSEHIISEVP KNVLIKSRML PDIFVEPSLV IEVLGSEITE SPSHTAGQGE GDTGLALRFP RFLRIRHDKG PYDITTLTEV RNLKEGKSI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial
Tag Info
N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Protein Families
ATP-dependent DNA ligase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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