Abbreviation
Recombinant Rhesus macaque CD4 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Macaca mulatta CD4 at 2 μg/mL can bind Anti-CD4 recombinant antibody (CSB-RA004935MA3HU).The EC50 is 2.057-2.679 ng/mL.
Alternative Names
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4;EGK_03289
Species
Macaca mulatta (Rhesus macaque)
Expression Region
26-396aa
Target Protein Sequence
KKVVLGKKGDTVELTCNASQKKNTQFHWKNSNQIKILGIQGSFLTKGPSKLSDRADSRKSLWDQGCFSMIIKNLKIEDSDTYICEVENKKEEVELLVFGLTANSDTHLLEGQSLTLTLESPPGSSPSVKCRSPGGKNIQGGRTISVPQLERQDSGTWTCTVSQDQKTVEFKIDIVVLAFQKASSTVYKKEGEQVEFSFPLAFTLEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQFQENLTCEVWGPTSPKLTLSLKLENKGATVSKQAKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVVPTWPTPVQP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Macaca mulatta CD4 at 2 μg/ml can bind Anti-CD4 recombinant antibody (CSB-RA004935MA3HU).The EC50 is 2.057-2.679 ng/mL.
Description
CD4 serves as the primary receptor for HIV envelope glycoprotein gp120 and plays a central role in T-cell activation, making it a critical target for studying viral entry mechanisms and immunomodulatory therapies in non-human primate models. This protein, spanning residues 26–396 and expressed in mammalian cells to preserve native glycosylation and disulfide bonding patterns, demonstrates quantifiable binding activity with an EC50 of 2.057–2.679 ng/mL against an anti-CD4 recombinant antibody in functional ELISA, providing a validated basis for therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody development. The defined binding kinetics support its use in surface plasmon resonance and biolayer interferometry experiments for affinity characterization of CD4-targeted biologics and small-molecule inhibitors. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein meets the quality thresholds commonly required for ligand-receptor interaction studies and serves as a positive control in binding assays evaluating immunotherapeutic candidates.
