Abbreviation
Recombinant Rhesus macaque F11 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 at 2 μg/mL can bind Anti-F11 recombinant antibody (CSB-RA007916MA1HU). The EC50 is 0.5654-0.6444 ng/mL.
②Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >150 pmol/min/µg.
Alternative Names
Coagulation factor XI; EGK_16300
Species
Macaca mulatta (Rhesus macaque)
Expression Region
19-624aa
Target Protein Sequence
ECVTRLFKDIHFEGGDIATVFTPSAKHCQVVCTHHPRCLLFTFTGESASEDPTQWFTCVLKDSVTETLPRVNRTGAISGYSFKQCSHQISACNKDIYVDLDMKGINYNSSLATSAQECQERCTDDVHCHFFTYATRQFPSLEHRNICLLKHTQTGTPTGIMKLDKVVTGFSLKSCALSNLACIRDVFPNTVFADSNIDSVMAPDAFVCRRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPRTRIKKSKALSGFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAVKGHEACQKLCTNAVRCQFFTYAPAQASCNEGKGKCYLKLSSNGSPTKILRGTGGISGYTLRLCKMDNECTTKIKPRIVGGTASVRGEWPWQVTLHTTSPTQRHLCGGSIIGNQWILTAAHCFYGVESPKILRVYIGILNQSEIKEDTSFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYTDSQRPICLPSKGDRNVIYTDCWVTGWGYRKLRDKIQNTLQKAKIPLVTNDECQKRYRGHKITHKMICAGYREGGKDACKGDSGGPLSCKHNEVWHLVGITSWGEGCAQRERPGVYTNVLEYVDWILEKTQA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 at 2 μg/ml can bind Anti-F11 recombinant antibody (CSB-RA007916MA1HU). The EC50 is 0.5654-0.6444 ng/mL.
-
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >150 pmol/min/µg.
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The purity of TrEMBL was greater than 95% as determined by SEC-HPLC
Description
Factor XI occupies a critical node in the intrinsic coagulation pathway, and this full-length mature protein (aa 19–624) demonstrates robust catalytic function with a specific activity exceeding 150 pmol/min/µg against the fluorogenic substrate Boc-IEGR-AMC, providing a quantitative foundation for kinetic parameter analysis including Km, Vmax, and kcat/Km determination. The protein exhibits an EC50 of 0.5654–0.6444 ng/mL in antibody-binding ELISA, confirming proper folding and epitope accessibility that supports its use as a positive control in immunoassays and as a reference standard in inhibitor screening campaigns aimed at identifying anticoagulant drug candidates. Dual purity verification by SDS-PAGE and SEC-HPLC (both >95%), combined with endotoxin levels below 1.0 EU/µg, meets the quality thresholds commonly required for accurate IC50 determination and substrate specificity profiling where contaminants would confound enzymatic measurements. The C-terminal His tag preserves active-site integrity, enabling structure-function studies and mechanism-of-action investigations in cardiovascular research models.
