Recombinant Macaca fascicularis albumin (ALB)

Thanks for your inquiry
1. This is a protein we successfully developed before. The current protein is with N-terminal 6xHis-tagged and expressed in yeast system which can satisfy your requirement.
2. We could perform Endotoxin Removal free of charge and guarantee endotoxin level <1.0 EU/ug of the protein by the LAL method.
This endotoxin removal is semi-quantitative, we can guarantee that the endotoxin level is less than 1.0 EU/ug, but we cannot determine a specific value.
Endotoxin (LPS) is mainly present in Gram-negative bacteria, which is the main constituent of the outer membrane of the cell wall, so it is mainly targeted for E. coli recombinant expression system.
Theoretically, there should exist no endotoxin in other recombinant expression systems.
However, laboratory reagents (including deionized water) and instruments may be affected by some endotoxin. The endotoxin content produced by this reason should be not too high in itself,
and it may be able to meet the needs of the customer, but we cannot guarantee it.
3. This protein has a his tag. The separation and purification of our protein is performed by Ni column affinity chromatography. The size of the band is detected by SDS-PAGE.
1) During the production process, we will perform sequencing in the cloning stage to ensure that the sequence is 100% correct (we can also provide sequencing reports if necessary).
2) After protein expression, SDS-PAGE is used to detect the band size, which is in line with the theoretical value.
3) We have a comprehensive purity of 90% or more by SDS-PAGE Coomassie blue staining and Bandscan software analysis.
4) You can perform SDS-PAGE test, and if available, mass spectrometry.