Abbreviation
Recombinant Cynomolgus monkey ADAM9 protein, partial, Biotinylated (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Anti-ADAM9 recombinant antibody(CSB-RA618774MA1HU) at 2 μg/mL can bind Macaca fascicularis ADAM9.The EC50 is 114.4-171.1 ng/mL.
Alternative Names
ADAM metallopeptidase domain 9
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
29-698aa
Target Protein Sequence
AARPGFQQTSHLSSYEIITPWRLTRERREAPRPYSKQVSYLIQAEGKEHIIHLERNKDLLPEDFVVYTYNKEGTVITDHPNIQNHCHFRGYVEGVYNSSVALSNCFGLRGLLHLENASYGIEPLQNSSHFEHIIYRMDDVHKEPLKCGVSNKDIEKETTKDEEEEPPSMTQLLRRRRAVLPQTRYVELFIVVDKERYDMMGRNQTAVREEMILLANYLDSMYIMLNIRIVLVGLEIWTNGNLINIAGGAGDVLGNFVQWREKFLITRRRHDSAQLVLKKGFGGTAGMAFVGTVCSRSHAGGINVFGHITVETFASIVAHELGHNLGMNHDDGRDCSCGAKSCIMNSGASGSRNFSSCSAEDFEKLTLNKGGNCLLNIPKPDEAYSAPSCGNKLVDAGEECDCGTPKECELDPCCEGSTCKLKSFAECAYGDCCKDCRFLPGGTLCRGRTSECDVPEYCNGSSQFCQPDVFIQNGYPCQNNKAYCYNGMCQYYDAQCQVIFGSKAKAAPKDCFIEVNSKGDRFGNCGFSGNEYKKCATGNALCGKLQCENVQEIPVFGIVPAIIQTPSRGTKCWGVDFQLGSDVPDPGMVNEGTKCGADKICRNFQCVDASVLNYDCDIQKKCHGHGVCNSNKNCHCENGWAPPNCETKGYGGSVDSGPTYNEMNTALRDG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-Avi-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
Measured by its binding ability in a functional ELISA.Immobilized Anti-ADAM9 recombinant antibody(CSB-RA618774MA1HU) at 2 μg/ml can bind Macaca fascicularis ADAM9.The EC50 is 114.4-171.1 ng/mL.
Description
ADAM9 metallopeptidase plays critical roles in ectodomain shedding and cell-surface protein processing, making it a key target in neuroscience research focused on synaptic remodeling and neuroinflammation. This cynomolgus macaque ortholog, spanning residues 29–698 with C-terminal His-Avi tags, demonstrates specific binding activity with an EC50 of 114.4–171.1 ng/mL in functional ELISA, confirming proper folding and epitope accessibility that supports its use in antibody-based detection assays and as a positive control in enzyme-linked screening platforms. The >95% purity determined by SDS-PAGE, combined with endotoxin levels below 1.0 EU/μg, meets the quality thresholds commonly required for inhibitor screening, IC50 determination, and drug candidate evaluation where contaminants could confound dose-response measurements. Biotinylation enables straightforward immobilization in surface plasmon resonance and biolayer interferometry experiments, facilitating kinetic parameter analysis and substrate specificity profiling without compromising the catalytic domain.
