Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.672-2.181 ng/mL.
Alternative Names
(Wnt-activated inhibitory factor 1)(WAIF1)
Molecular Characterization
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
35-355aa
Target Protein Sequence
TSSASSSSSSAPFLASAASAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPLYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRGGAFEHLPSLRQLDLSHNPLAYLSPFAFSGSNASISAPSPLVELILNHIVPPDDKRQNRSFEGMVAAALVAGRALQGLHLLELASNHFLYLPRDVLAQLPSLRYLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHVRVFLDNNPWVCDCHMADMVTWLKQTGVVQGKDRLTCAFPEKMRNRVLLELNSADLDCDPILPPSLQTS
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.672-2.181 ng/mL.
Description
This Macaca fascicularis TPBG recombinant protein was produced in mammalian cell, where the gene sequence encoding Macaca fascicularis TPBG (35-355aa) was expressed with the N-terminal 10xHis tag and C-terminal Myc tag. The purity of this TPBG protein was greater than 95%. The activity was tested.
TPBG is expressed on a variety of malignancies, but has limited expression in normal adult tissues. At present, the global TPBG research pipeline is not very dense, and it is scattered in various drug types (vaccine, ADCs, bispecific antibodies, fusion protein, etc.). Drug candidates targeting TPBG have demonstrated good preclinical antitumor activity and safety, indicating that TPBG is a very attractive target in tumor immunotherapy. The preparation of Macaca fascicularis TPBG recombinant protein can help its related drug development and research.
