Recombinant Influenza A virus Polymerase acidic protein (PA), partial

Amino acids 124-247 constitute the expression domain of recombinant Influenza A virus PA (Polymerase acidic protein). This PA (Polymerase acidic protein) protein is theoretically predicted to have a molecular weight of 18.6 kDa. The PA (Polymerase acidic protein) protein was expressed in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of PA (Polymerase acidic protein), making it easier to detect and purify the PA (Polymerase acidic protein) recombinant protein in the later stages of expression and purification.

The influenza A virus polymerase acidic protein (PA) is a crucial component of the viral RNA polymerase complex responsible for transcribing and replicating the viral genome. PA, along with polymerase basic proteins (PB1 and PB2), forms the heterotrimeric RNA-dependent RNA polymerase essential for viral replication and transcription. PA possesses endonuclease activity, cleaving host cell mRNA to generate primers for viral RNA synthesis. This cap-snatching mechanism is vital for initiating viral transcription. Additionally, PA plays a role in regulating the switch from viral RNA transcription to replication. Understanding the functions of the influenza A virus PA protein is critical for developing antiviral strategies and vaccines targeting the viral polymerase complex.