Recombinant Influenza A virus Nucleoprotein (NP)

CSB-YP325878IMI

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Review （1）

Size:

20ug100ug1mg(1mg*1 or 500ug*2)

US$436

Quantity:

Express system: Yeast

Species: Influenza A virus (strain A/Equine/Prague/1/1956 H7N7)

Tag Info: N-terminal 6xHis-tagged

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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Uniprot NO.

P16980

Research Area

Others

Species

Influenza A virus (strain A/Equine/Prague/1/1956 H7N7)

Source

Yeast

Expression Region

1-498aa

Target Protein Sequence

MASQGTKRPYEQMETGGERQNATEIRASVGKMVGGIGRFYIQMCTELKLNDYEGRLIQNSITIEKMVLSAFDERRNKYLEEHPNTGKDPKKTGGPIYRKREGKWIRELILYDKEEIRRIWRQANNGEDATAGLTHLMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLIVASGYDFEREGYSLVGVDPFKLLQNSQIFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTKVIPRGQLSTRGIQIASNENMETIDSNTLELRSRYWAIRTKSGGNTSQQKASAGQISVQPTFSVQRNLPFERTTIMAAFTGNNEGRTSDMRTEIIRMMENAKPDDVSFQGRGVFELSDEKATNPIVPSFDMSKEGSYFFGDNAEEFDN

Mol. Weight

58.2 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

Please contact us to get it.

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Description

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■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : a:1:{i:0;a:2:{i:0;s:48:" ELISA for detection of serum anti-NP antibodies";i:1;s:0:"";}}

Review: describe

By Anonymous

Target Background

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Subcellular Location

Virion. Host nucleus.

Protein Families

Influenza viruses nucleoprotein family

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