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Recombinant Human herpesvirus 1 Tegument protein VP16 (UL48)

Recombinant Human herpesvirus 1 Tegument protein VP16 (UL48)

CSB-EP356268HWZ

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Size:

20ug100ug1mg(1mg*1 or 500ug*2)

US$224

Quantity:

Express system: E.coli

Species: Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)

Tag Info: N-terminal 6xHis-SUMO-tagged

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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

UL48

Uniprot NO.

P04486

Research Area

Others

Alternative Names

Alpha trans-inducing protein Alpha-TIF ICP25 Vmw65

Species

Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)

Source

E.coli

Expression Region

1-490aa

Target Protein Sequence

MDLLVDELFADMDADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYVPERAQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILWAAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIREHLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMREHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG

Mol. Weight

70.3 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Datasheet & COA

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Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356268HWZ could indicate that this peptide derived from E.coli-expressed Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1) UL48.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356268HWZ could indicate that this peptide derived from E.coli-expressed Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1) UL48.

Description

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Target Background

Function

Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection.; May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.

Subcellular Location

Virion tegument. Host nucleus.

Protein Families

Herpesviridae tegument protein VP16 protein family

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