Home
Product
Protein
Recombinant Proteins
Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5), partial

Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

HELI

Uniprot NO.

P10189

Alternative Names

HELI; UL5DNA replication helicase; EC 3.6.4.-

Species

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)

Source

Yeast

Expression Region

1-882

Target Protein Sequence

MAAAGGERQL DGQKPGPPHL QQPGDRPAVP GRAEAFLNFT SMHGVQPILK RIRELSQQQL DGAQVPHLQW FRDVAALESP AGLPLREFPF AVYLITGNAG SGKSTCVQTI NEVLDCVVTG ATRIAAQNMY AKLSGAFLSR PINTIFHEFG FRGNHVQAQL GQYPYTLTSN PASLEDLQRR DLTYYWEVIL DLTKRALAAS GGEELRNEFR ALAALERTLG LAEGALTRLA PATHGALPAF TRSNVIVIDE AGLLGRHLLT AVVYCWWMIN ALYHTPQYAA RLRPVLVCVG SPTQTASLES TFEHQKLRCS VRQSENVLTY LICNRTLREY ARLSYSWAIF INNKRCVEHE FGNLMKVLEY GLPITEEHMQ FVDRFVVPEN YITNPANLPG WTRLFSSHKE VSAYMAKLHA YLKVTREGEF VVFTLPVLTF VSVKEFDEYR RLTHQPGLTI EKWLTANASR ITNYSQSQDQ DAGHMRCEVH SKQQLVVARN DVTYVLNSQI AVTARLRKLV FGFSGTFRAF EAVLRDDSFV KTQGETSVEF AYRFLSRLIF SGLISFYNFL QRPGLDATQR TLAYARMGEL TAEILSLRPK SSGVPTQASV MADAGAPGER AFDFKQLGPR DGGPDDFPDD DLDVIFAGLD EQQLDVFYCH YTPGEPETTA AVHTQFALLK RAFLGRFRIL QELFGEAFEV APFSTYVDNV IFRGCEMLTG SPRGGLMSVA LQTDNYTLMG YTYARVFAFA DELRRRHATA NVAELLEEAP LPYVVLRDQH GFMSVVNTNI SEFVESIDST ELAMAINADY GISSKLAMTI TRSQGLSLDK VAICFTPGNL RLNSAYVAMS RTTSSEFLRM NLNPLRERHE RDDVISEHIL SALRDPNVVI VY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life of liquid-form form is 6 months around at -20°C/-80°C.
The shelf life of lyophilized form is 12 months around at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Possesses helicase-like motifs and therefore may act as the helicase subunit of the complex.

Gene References into Functions

Data indicate that the N-terminal domain of UL52 primase binds UL5 helicase and the middle domain interacts with Ul8. PMID:25278021
Results showed that the UL29 and UL5 transgenes are stable in that there are one and three copies of the UL29 and UL5 genes, respectively, per cell in both the AV529-19 MCB and ECB. PMID:24140636
HSV-1 single stranded DNA binding protein (ICP8), and the helicase/primase complex (UL8/UL5/UL52) form a nuclear complex in transfected cells that is necessary and sufficient to disable ATR signaling. PMID:24098119
Taken together, these data suggest that the primase activity of the helicase-primase requires formation of a dimer or higher-order structure while ATPase activity does not. PMID:21068246
We present evidence that single mutations close to a predicted functional domain of an essential HSV-1 replication enzyme (helicase) are associated with drug resistance and virus growth characteristics. PMID:17404685
We believe our report to be the first to describe an HPI(helicase-primase inhibitors)-resistant HSV-1 UL5 mutant, that is markedly less virulent in vivo and slowly growing in tissue culture compared to the parental strain. [UL5] PMID:18539344
Slower growth and moderately reduced virulence suggest that the Asn342Lys mutation might interferes with the helicase-primase activity. PMID:19403757
Herpes simplex virus-1 DNA primase does not absolutely need to form Watson-Crick hydrogen bonds to efficiently polymerize a NTP. The enzyme can misincorporate nucleotides both by misreading the template and by a primer-template slippage mechanism. PMID:19835416