Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Adhesion
Alternative Names
THBS 1; THBS; Thbs1; Thrombospondin 1; Thrombospondin-1; TSP 1; TSP; TSP1; TSP1_HUMAN
Species
Homo sapiens (Human)
Expression Region
19-344aa
Target Protein Sequence
NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
The production of this Recombinant Human THBS1 protein started with the THBS1 gene synthesis. And then using recombinant DNA technology, the THBS1 gene was inserted into an expression vector so that we could get the recombinant express plasmid of THBS1. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human THBS1 protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE.
THBS1 (also named TSP or TSP1) is a gene providing an instruction of making a protein named thrombospondin-1 (abbreviated as THBS1) in human. THBS1 protein is also called glycoprotein G and is a subunit of a disulfide-linked homotrimeric protein. This protein is well known as a natural angiogenesis inhibitor and has a potent effect on tumor growth of the ability to inhibit angiogenesis. The anti-angiogenic effect of thrombospondin-1 is mediated by inhibiting vascular endothelial cell adhesion and migration.
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