Abbreviation
Recombinant Human TIGIT protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
FACS assay shows that Human TIGIT can bind to 293F cell overexpressing human CD155.
Alternative Names
(VSIG9)(VSTM3)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
22-141aa
Target Protein Sequence
MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal hFc1-Myc-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
FACS assay shows that Human TIGIT can bind to 293F cell overexpressing human CD155.
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The purity of Human TIGIT was greater than 95% as determined by SEC-HPLC
Description
TIGIT functions as a key inhibitory receptor in the immune checkpoint axis, competing with CD226 for binding to CD155 (PVR) and suppressing T-cell and NK-cell activation. This recombinant construct covers the extracellular immunoglobulin domain (aa 22–141) with a C-terminal hFc1-Myc tag, and its biological activity has been confirmed by FACS demonstrating direct binding to human CD155-overexpressing 293F cells — providing a validated positive control for receptor-ligand interaction assays, competitive inhibition studies, and blocking antibody screening. Mammalian cell expression preserves native glycosylation and disulfide-mediated folding critical for conformational integrity of the IgV domain, making this protein appropriate for therapeutic antibody epitope mapping, affinity characterization by SPR or BLI, and small-molecule inhibitor screening in immune checkpoint drug discovery programs. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based functional assays and sensitive biophysical binding measurements.
