Abbreviation
Recombinant Human SDC1 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA.Immobilized Human SDC1 at 2 μg/mL can bind anti-SDC1 recombinant antibody(CSB-RA020888MA1HU).The EC50 is 0.7196-0.8309 ng/mL. ②SDC1 Recombinant Monoclonal Antibody(CSB-RA020888MA1HU) captured on Protein A Chip can bind Human SDC1 with an affinity constant of 6.79 nM as detected by MetaSPR Assay (WeSPRTM 200).
Alternative Names
Syndecan-1; SYND1; CD antigen CD138
Species
Homo sapiens (Human)
Expression Region
23-254aa
Target Protein Sequence
QIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human SDC1 at 2 μg/ml can bind anti-SDC1 recombinant antibody(CSB-RA020888MA1HU).The EC50 is 0.7196-0.8309 ng/mL.
-
Activity
SDC1 Recombinant Monoclonal Antibody(CSB-RA020888MA1HU) captured on Protein A Chip can bind Human SDC1 with an affinity constant of 6.79 nM as detected by MetaSPR Assay (WeSPRTM 200).
Description
Syndecan-1 functions as a critical co-receptor in cell–matrix and cell–cell adhesion events, making it a key target in cancer metastasis and immune surveillance research. This mammalian-expressed construct spanning residues 23–254 captures the complete extracellular domain, preserving native glycosylation patterns essential for physiological ligand interactions. Functional validation demonstrates robust antibody binding with an EC50 of 0.72–0.83 ng/mL in ELISA and a binding affinity of 6.79 nM by surface plasmon resonance, confirming that the protein adopts a conformation suitable for antibody development and epitope mapping studies. With purity exceeding 95% and endotoxin levels below 1.0 EU/μg, this reagent meets the quality thresholds commonly required for cell adhesion assays, tumor cell migration experiments, and integrin-ligand binding studies where low endotoxin is critical to avoid confounding inflammatory responses.
