Abbreviation
Recombinant Human SIRPB1 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA.Immobilized Human SIRPB1 at 2 μg/mL can bind anti-SIRPB1 recombinant antibody(CSB-RA021335MA1HU).The EC50 is 0.9675-1.114 ng/mL. ②SIRPB1 Recombinant Monoclonal Antibody(CSB-RA021335MA1HU) captured on Protein A Chip can bind Human SIRPB1 with an affinity constant of 0.245 nM as detected by MetaSPR Assay (WeSPRTM 200).
Alternative Names
Signal-regulatory protein beta-1; SIRP-beta-1; CD172 antigen-like family member B; CD antigen CD172b
Species
Homo sapiens (Human)
Expression Region
30-371aa
Target Protein Sequence
EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human SIRPB1 at 2 μg/ml can bind anti-SIRPB1 recombinant antibody(CSB-RA021335MA1HU).The EC50 is 0.9675-1.114 ng/mL.
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Activity
SIRPB1 Recombinant Monoclonal Antibody(CSB-RA021335MA1HU) captured on Protein A Chip can bind Human SIRPB1 with an affinity constant of 0.245 nM as detected by MetaSPR Assay (WeSPRTM 200).
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The purity of SIRPB1 was greater than 95% as determined by SEC-HPLC
Description
SIRPB1 functions as an immunomodulatory receptor on myeloid and lymphoid cells, and this mammalian-expressed extracellular domain (aa 30–371) preserves the native glycosylation and disulfide architecture required for physiologically relevant antibody recognition. Functional ELISA demonstrates dose-dependent binding to an anti-SIRPB1 recombinant antibody with an EC50 of 0.97–1.11 ng/mL, while surface plasmon resonance confirms high-affinity interaction at a KD of 0.245 nM, providing quantitative benchmarks for therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody validation in immune checkpoint research. The dual-method purity confirmation (>95% by SDS-PAGE and SEC-HPLC) combined with endotoxin levels below 1.0 EU/μg aligns with standards expected in ligand-binding assays, SPR-based affinity characterization, and biolayer interferometry studies where contaminant interference must be minimized. This partial construct serves as a positive control in receptor-ligand interaction platforms and supports drug discovery workflows targeting SIRP family members in cancer immunotherapy.
