Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
C358B7.1; p18; SUMO 1 protein ligase; SUMO conjugating enzyme UBC9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO1 protein ligase; UBC9; UBC9_HUMAN; UBCE9; Ube2i; Ubiquitin carrier protein 9; Ubiquitin carrier protein; Ubiquitin carrier protein I; Ubiquitin conjugating enzyme 9; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9); Ubiquitin conjugating enzyme E2I (UBC9 homolog; yeast); Ubiquitin conjugating enzyme UbcE2A; Ubiquitin like protein SUMO 1 conjugating enzyme; Ubiquitin protein ligase E2I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I
Species
Homo sapiens (Human)
Expression Region
1-157aa
Target Protein Sequence
MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal GST-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO synthesized the recombinant gene by integrating the N-terminal GST tag sequence into the targeted gene encoding the 1-157aa of the human UBE2I. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant human UBE2I protein carrying N-terminal GST tag. The SDS-PAGE assayed the purity of this recombinant UBE2I protein greater than 90%. This UBE2I protein migrated along the gel to a band of about 45 kDa molecular weight.
UBE2I is a gene providing instruction of making a protein named SUMO-conjugating enzyme UBC9 (also called Ubiquitin-protein ligase I and abbreviated as p18) in human and belongs to Ubiquitin-conjugating enzyme family. SUMO is an abbreviated form of small ubiquitin-like modifiers. SUMOylation is a process of covalent and reversible attaching of SUMO moiety to a target protein and is an important post-translational modification involved in various cellular processes. UBC9 is the unique SUMO E2 enzyme known ro conjugate SUMO to target substrates. The UBC9 serves as a lynchpin in the SUMO conjugateion pathway, interecting with the SUMO E1 during activation.
