Recombinant Human Protein Wnt-2b (WNT2B)

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Code: CSB-CF856613HU

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US$878

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Express system: in vitro E.coli expression system

Species: Homo sapiens (Human)

Tag Info: N-terminal 6xHis-SUMO-tagged

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Target Background

Product Details

Abbreviation

Recombinant Human WNT2B protein

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

WNT2B

Uniprot NO.

Q93097

Research Area

StemCells

Species

Homo sapiens (Human)

Source

in vitro E.coli expression system

Expression Region

1-391aa

Target Protein Sequence

MLRPGGAEEAAQLPLRRASAPVPVPSPAAPDGSRASARLGLACLLLLLLLTLPARVDTSWWYIGALGARVICDNIPGLVSRQRQLCQRYPDIMRSVGEGAREWIRECQHQFRHHRWNCTTLDRDHTVFGRVMLRSSREAAFVYAISSAGVVHAITRACSQGELSVCSCDPYTRGRHHDQRGDFDWGGCSDNIHYGVRFAKAFVDAKEKRLKDARALMNLHNNRCGRTAVRRFLKLECKCHGVSGSCTLRTCWRALSDFRRTGDYLRRRYDGAVQVMATQDGANFTAARQGYRRATRTDLVYFDNSPDYCVLDKAAGSLGTAGRVCSKTSKGTDGCEIMCCGRGYDTTRVTRVTQCECKFHWCCAVRCKECRNTVDVHTCKAPKKAEWLDQT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

59.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

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Datasheet & COA

Please contact us to get it.

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Description

The expression region of this recombinant Human WNT2B covers amino acids 1-391. The expected molecular weight for the WNT2B protein is calculated to be 59.8 kDa. Expression of this WNT2B protein is conducted in in vitro e.coli expression system. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of WNT2B, making it easier to detect and purify the WNT2B recombinant protein in the later stages of expression and purification.

The protein Wnt-2b (WNT2B) is a highly researched protein with primary areas of investigation spanning developmental biology, cell signaling, and tumorigenesis. In developmental biology, WNT2B, as a member of the Wnt family, plays a crucial role in embryonic development and organ formation, particularly in the development of the nervous system and muscle tissues. In cell signaling, WNT2B regulates various cellular functions, including proliferation, differentiation, and migration, through the Wnt signaling pathway. In the context of tumor development, the aberrant expression of WNT2B is associated with the occurrence and progression of multiple cancers, making it an important target in cancer biology research.

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Target Background

Function(From Uniprot)

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.

Gene References into Functions

Wnt2B co-operates with Frizzled7 to mediate mesenchymal to epithelial transition in colorectal cancer. PMID:28560804
Altered WNT2B expression in bladder wall fibroblasts alters expression of the apoptosis inductor TNFSF10. PMID:29131138
we identified two independent SNPs (i.e., WNT2B rs1175649 G>T and BTRC rs61873997 G>A) that showed a predictive role in CM-specific survival, with an effect-allele-attributed hazards ratio (adjusted hazards ratio) of 1.99 (95% confidence interval = 1.41-2.81, P = 8.10 x 10(-5)) and 0.61 (0.46-0.80, 3.12x10(-4)), respectively. PMID:28499756
A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively). PMID:27793913
ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis. PMID:25356045
these results indicate that the high-expression levels of Gli1 and Wnt2B might play a pivotal role during tumorigenesis of pancreatic cancer PMID:25120849
Results showed that miR-185-3p contributs to radioresistance of nasopharyngeal carcinoma via modulation of WNT2B expression. PMID:25297925
secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism PMID:23785285
High Wnt2B overexpression is associated with ovarian cancer metastasis and drug resistance. PMID:22635028
This is the first report on differential regulation of WNT2 and WNT2B mRNAs in human cancer cell lines. PMID:11712082
Wnt-2b mRNA is expressed in human ovarian cancer cells PMID:12072409
REVIEW: Anti-WNT2B monoclonal antibodies, WNT2B RNAi compounds, or small molecule WNT2B inhibitors could be developed as novel therapeutic agents for gastric cancer and esophageal cancer in the field of clinical oncology. PMID:16273293
in addition to alternative promoters and RNA splicing, an alternative translation start in Wnt13B and Wnt13C mRNAs increases the complexity of both human wnt13 expression and functions PMID:16407296
Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3. PMID:17649721
Hedgehog signals and bHLH transcription factors are involved in WNT2B upregulation, which is counteracted by BMP, WNT and Notch signals. PMID:19360354

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Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted.

Protein Families

Wnt family

Tissue Specificity

Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidne

Database Links

HGNC: 12781

UNIGENE: Hs.258575

KEGG: hsa:7482

STRING: 9606.ENSP00000358698

OMIM: 601968

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