Abbreviation
Recombinant Human WNT2B protein
Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
WNT2B; WNT13; Protein Wnt-2b; Protein Wnt-13
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
1-391aa
Target Protein Sequence
MLRPGGAEEAAQLPLRRASAPVPVPSPAAPDGSRASARLGLACLLLLLLLTLPARVDTSWWYIGALGARVICDNIPGLVSRQRQLCQRYPDIMRSVGEGAREWIRECQHQFRHHRWNCTTLDRDHTVFGRVMLRSSREAAFVYAISSAGVVHAITRACSQGELSVCSCDPYTRGRHHDQRGDFDWGGCSDNIHYGVRFAKAFVDAKEKRLKDARALMNLHNNRCGRTAVRRFLKLECKCHGVSGSCTLRTCWRALSDFRRTGDYLRRRYDGAVQVMATQDGANFTAARQGYRRATRTDLVYFDNSPDYCVLDKAAGSLGTAGRVCSKTSKGTDGCEIMCCGRGYDTTRVTRVTQCECKFHWCCAVRCKECRNTVDVHTCKAPKKAEWLDQT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human WNT2B covers amino acids 1-391. The expected molecular weight for the WNT2B protein is calculated to be 59.8 kDa. Expression of this WNT2B protein is conducted in in vitro e.coli expression system. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of WNT2B, making it easier to detect and purify the WNT2B recombinant protein in the later stages of expression and purification.
The protein Wnt-2b (WNT2B) is a highly researched protein with primary areas of investigation spanning developmental biology, cell signaling, and tumorigenesis. In developmental biology, WNT2B, as a member of the Wnt family, plays a crucial role in embryonic development and organ formation, particularly in the development of the nervous system and muscle tissues. In cell signaling, WNT2B regulates various cellular functions, including proliferation, differentiation, and migration, through the Wnt signaling pathway. In the context of tumor development, the aberrant expression of WNT2B is associated with the occurrence and progression of multiple cancers, making it an important target in cancer biology research.
