Abbreviation
Recombinant Human PCSK9 protein (D374Y), Biotinylated (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Anti-PCSK9 recombinant antibody (CSB-RA017647MA2HU) at 2 μg/mL can bind Human PCSK9 protein. The EC50 is 7.450-8.692 ng/mL.
②Measured by its binding ability in a functional ELISA. Immobilized Human PCSK9 at 1 μg/mL on streptavidin coated plates can bind Anti-PCSK9 recombinant antibody (CSB-RA017647MA2HU) . The EC50 is 0.3792-1.157 ng/mL.
Research Area
Cardiovascular
Alternative Names
Proprotein convertase subtilisin/kexin type 9; EC:3.4.21.-; Neural apoptosis-regulated convertase 1 (NARC-1); Proprotein convertase 9 (PC9); Subtilisin/kexin-like protease PC9; PCSK9; NARC1; PSEC0052
Species
Homo sapiens (Human)
Expression Region
31-692aa(D374Y)
Target Protein Sequence
QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSYCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-Avi-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Anti-PCSK9 recombinant antibody (CSB-RA017647MA2HU) at 2 μg/ml can bind Human PCSK9 protein. The EC50 is 7.450-8.692 ng/mL.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PCSK9 at 1 μg/ml on streptavidin coated plates can bind Anti-PCSK9 recombinant antibody (CSB-RA017647MA2HU) . The EC50 is 0.3792-1.157 ng/mL.
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The purity of PCSK9 was greater than 95% as determined by SEC-HPLC
Description
PCSK9 regulates cholesterol homeostasis by promoting degradation of the LDL receptor, and the D374Y gain-of-function mutation intensifies this interaction, making it a critical tool for studying hypercholesterolemia mechanisms and therapeutic intervention strategies. This full-length variant (aa 31–692) demonstrates robust binding activity in functional ELISA, achieving an EC50 of 7.450–8.692 ng/mL when captured by immobilized anti-PCSK9 antibody and an EC50 of 0.3792–1.157 ng/mL in the reverse orientation with immobilized biotinylated protein binding soluble antibody, providing quantitative benchmarks for inhibitor screening and IC50 determination in drug candidate evaluation. The C-terminal His-Avi tag enables oriented immobilization via streptavidin without interfering with the catalytic domain, supporting kinetic parameter analysis and substrate specificity profiling where precise orientation is essential. Purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg satisfy the quality thresholds commonly required for enzymatic activity assays and structural studies including crystallography.
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